卵形鲳鲹对饲料中泛酸的需求量  

Dietary pantothenic acid requirement of juvenile golden pompano(Trachinotus ovatus)

作　　者：荀鹏伟 林黑着[1,3] 黄忠 周传朋[1] 杨育凯 虞为[1,3] 黄倩倩[1,2] 王珺 王芸[1] 谭连杰 林益坤 XUN Pengwei;LIN Heizhao;HUANG Zhong;ZHOU Chuanpeng;YANG Yukai;YU Wei;HUANG Qianqian;WANG Jun;WANG Yun;TAN Lianjie;LIN Yikun(Key Laboratory of South China Sea Fishery Resources Exploitation&Utilization,Ministry of Agriculture and Rural Affairs;South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510300,China;College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201506,China;Shenzhen Base of South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Shenzhen 518121,China;College of Life Sciences,Guangzhou University,Guangzhou 510006,China)

机构地区：[1]中国水产科学研究院南海水产研究所,农业农村部南海渔业资源开发利用重点实验室,广东广州510300 [2]上海海洋大学水产与生命学院,上海201506 [3]中国水产科学研究院南海水产研究所深圳试验基地,广东深圳518121 [4]广州大学生命科学学院,广东广州510006

出　　处：《南方水产科学》2018年第5期80-87,共8页South China Fisheries Science

基　　金：中国水产科学研究院南海水产研究所中央级公益性科研院所基本科研业务费专项资金资助(2017ZD01);国家自然科学基金项目(31602175);深圳市战略性新兴产业发展专项资金(SWCYL20150330010013);中国水产科学研究院基本科研业务费资助(2018HYZD0506);广州市科技计划项目(201707010445)。

摘　　要：挑选体质量为(8.80±0.10) g的卵形鲳鲹(Trachinotus ovatus)幼鱼,随机分成6组,每组3个平行,每个平行20尾,放于池塘网箱中,分别饲喂泛酸质量分数为0、16.4 mg·kg^(–1)、20 mg·kg^(–1)、26 mg·kg^(–1)、33.4 mg·kg^(–1)和37 mg·kg^(–1)的半纯化饲料8周,每天饱食投喂2次,研究并确定其对泛酸的需求量。结果表明,饲料中添加适量泛酸可以显著提高卵形鲳鲹幼鱼增重率、特定生长率、血清补体C4体积分数和肠道中淀粉酶(AMS)、肌酸激酶(CK)和Na^+-K^+-ATP酶活性(P<0.05),并且随着饲料中泛酸质量分数的增加,其增重率、特定生长率、补体C4体积分数、AMS、CK和Na^+-K^+-ATP酶活性先升高后下降;33.4 mg·kg^(–1)泛酸组的肌肉蛋白含量显著高于未添加泛酸组和16.4 mg·kg^(–1)泛酸组(P<0.05);饲料中添加适量泛酸可以显著提高卵形鲳鲹血清中高密度胆固醇水平、溶菌酶(LZM)活性和肠道γ-谷氨酰转肽酶(γ-GT)活性(P<0.05),且随着泛酸水平的增加先升高后保持稳定。二次回归分析结果表明,以增重率为指标,卵形鲳鲹幼鱼对泛酸的最适需求量为21.03 mg·kg^(–1)饲料。The juvenile golden pompano[(8.80±0.10)g]were randomly assigned into six groups with three replicates in each group and 20 fish in a replicate.They had been fed with pantothenic acid mass fractions of 0,16.4 mg·kg^–1,20 mg·kg^–1,26 mg·kg^–1,33.4 mg·kg^–1 and 37 mg·kg^–1 in the cages twice a day for eight weeks.The results show that moderate pantothenic acid in feed had significant effects on the weight gain rate,specific growth rate,content of complement C4 in serum as well as activities of amylase(AMS),creatine kinase(CK)and Na+-K+-ATPase in intestine(P<0.05).They increased first and then decreased with increase of dietary pantothenic acid.In muscle,the protein content in 33.4 mg·kg^–1 group was significantly higher than that in the free-pantothenic acid group and 16.4 mg·kg^–1 group.The pantothenic acid level in the feed significantly affected the high density lipoprotein cholesterol(HDL-C),lysozyme(LZM)in the serum and activity ofγ-GT in the intestine(P<0.05).They increased first and then was stable.The quadratic regression analysis on the weight gain rate indicates that the recommended optimum dietary pantothenic acid level for optimal growth of juvenile golden pompano was 21.03 mg·kg^–1.

关 键 词：卵形鲳鲹 泛酸 需求量 生长性能 肠道消化吸收 

分 类 号：S963.731[农业科学—水产养殖]