4-羟苯基维胺对瘢痕疙瘩成纤维细胞Procollagen Ⅰ和MMP-1 mRNA表达的影响  

Effect of 4-hydroxyphenyl-retinamide on type ⅠProcollagen and MMP-1 mRNA expression in keloid fibroblasts

作　　者：南美兰[1] 金玟言 金哲虎[1] 金承龙[1] NAN Meilan;JIN Wenyan;JIN Zhehu;JIN Chenglong(Departmentof Dermatology,Affiliated Hospital of Yanbian University,Yanji 133000,China)

机构地区：[1]延边大学附属医院皮肤科,吉林延吉133000

出　　处：《实用医学杂志》2018年第19期3172-3175,共4页The Journal of Practical Medicine

基　　金：国家自然科学基金项目(编号:81260233);吉林省科技厅自然科学基金项目(编号:20160101183JC);延边大学科技计划项目青年科研基金项目[编号:(2015)第31号]

摘　　要：目的探讨4-羟苯基维胺(4-HPR)对瘢痕疙瘩成纤维细胞Ⅰ型前胶原(ProcollagenⅠ)和基质金属蛋白酶-1(MMP-1)mRNA表达的影响。方法运用实时荧光定量PCR方法测定瘢痕疙瘩成纤维细胞中ProcollagenⅠ和MMP-1 mRNA表达情况。观察4-HPR对ProcollagenⅠ和MMP-1 m RNA表达的影响。结果瘢痕疙瘩成纤维细胞中ProcollagenⅠmRNA表达明显高于正常皮肤成纤维细胞,而MMP-1 mRNA低表达,差异具有统计学意义。4-HPR不同浓度组瘢痕疙瘩成纤维细胞ProcollagenⅠmRNA表达水平明显减少,MMP-1 mRNA表达水平明显增加,差异具有统计学意义。结论 ProcollagenⅠ和MMP-1基因表达在瘢痕生成过程中起重要作用。4-HPR可抑制ProcollagenⅠ和促进MMP-1 mRNA表达水平。Objective To investigate the effect of 4-HPR onProcollagenⅠand MMP-1 mRNA expression in keloid fibroblasts.Methods The expression ofProcollagenⅠand MMP-1 mRNA was detected by real-time PCR in keloid fibroblasts.The expression of ProcollagenⅠand MMP-1 mRNA was treated by 4-HPR in keloid fibroblasts.Results The expression of ProcollagenⅠmRNA in keloid fibroblasts was significantly higher than that in the normal fibroblasts,while MMP-1 mRNA was significantly lower.Expression of ProcollagenⅠmRNA decreased and MMP-1 mRNA increased in 4-HPR group.Conclusion ProcollagenⅠand MMP-1 may play some roles in the regulation of keloid formation.4-HPR inhibits ProcollagenⅠexpression and promotes MMP-1 mRNA expression in keloid fibroblasts.

关 键 词：瘢痕疙瘩 4-羟苯基维胺 Ⅰ型前胶原 基质金属蛋白酶-1 

分 类 号：R622[医药卫生—整形外科]