N-糖基化蛋白质组样品富集策略的研究进展  

Research Advances of Enrichment Approaches in N-glycoproteomics

作　　者：邵文亚[1,2] 梁玉 梁振[1] 张丽华[1] 张玉奎[1] SHAO Wen-ya;LIANG Yu;LIANG Zhen;ZHANG Li-hua;ZHANG Yu-kui(Key Laboratory of Separation Science for Analytical Chemistry,Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian 116023,China;University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]中国科学院大连化学物理研究所中国科学院分离分析化学重点实验室,辽宁大连116023 [2]中国科学院大学,北京100049

出　　处：《分析测试学报》2018年第10期1212-1216,共5页Journal of Instrumental Analysis

基　　金：国家自然科学基金项目(91753110)

摘　　要：蛋白质的糖基化是生物体内重要的蛋白质翻译后修饰之一,但其丰度通常较低,糖基化蛋白质酶解肽段中仅有2%~5%为糖基化肽段,因此,为实现糖基化蛋白质组的深度覆盖分析,对糖基化蛋白质/肽段进行富集是非常必要的。该文对糖基化蛋白质组样品不同富集方法的原理、特点以及最新研究进展进行了综述,同时也对N-糖基化蛋白质组学富集策略的发展前景进行了展望。Protein glycosylation is one of the most important post-translational modifications.However,the great challenges for glycosylation of protein/peptides are the inherently low abundance and the poor ionization efficiency in mass spectrometry identification.Therefore,the specific enrichment before MS identification is indispensable.The basic principles,characteristics and recent progresses of the enrichment approaches for glycosylated proteins/peptides are reviewed,and the development prospects of the enrichment approaches for N-glycosylated proteins/peptides are also outlined.

关 键 词：糖蛋白质组学 N-糖基化 糖肽 糖蛋白 富集 

分 类 号：O629.73[理学—有机化学] G353.11[理学—化学]