Ru^(3+)增敏的化学发光新体系测定呋塞米的研究  

Investigation of a Novel Ru^(3+)-Sensitized Chemiluminescence System for Furosemide Determination

作　　者：于翔[1] 郗娟 YU Xiang;XI Juan(Institute of Environmental&Chemical Engineering,Xi an Polytechnic University,Xi an 710048,China;Shaanxi Tianan Environmental Protection Technology Co.,Ltd.,Xi an 710048,China)

机构地区：[1]西安工程大学环境与化学工程学院,陕西西安710048 [2]陕西天安环保科技有限公司,陕西西安710048

出　　处：《分析测试学报》2018年第9期1076-1081,共6页Journal of Instrumental Analysis

基　　金：陕西省教育厅专项科研计划(09JK779)

摘　　要：Ru^(3+)存在下,呋塞米能够大幅度增强三(1,10-菲咯啉)钌(Ⅱ)(Ru(phen)_3^(2+))-Ce(Ⅳ)体系的化学发光,且当体系中Ru^(3+)的浓度从0增至15μmol/L时,呋塞米对体系发光的增强值提高1个数量级,基于此,建立了高灵敏测定呋塞米的Ru(phen)_3^(2+)-Ce(Ⅳ)-Ru^(3+)体系化学发光方法。在优化实验条件下,该法测定呋塞米的线性范围为5. 0×10^(-9)~2. 0×10^(-6)mol/L,检出限为3. 8×10^(-9)mol/L。方法具有较高的分析灵敏度,将其应用于呋塞米片剂和呋塞米注射液的分析,结果满意。结合紫外光谱的研究结果,对化学发光反应机理进行了探讨。A novel Ru(phen)2+3-Ce(Ⅳ)-Ru^3+chemiluminescence(CL)method was developed for the determination of furosemide based on the phenomenon that in the presence of ruthenium(Ⅲ)(Ru^3+),furosemide could greatly enhance the CL intensity of tris-(1,10-phenanthroline)ruthenium(Ⅱ)(Ru(phen)2+3)-cerium(Ⅳ)(Ce(Ⅳ))system.With the increase of Ru^3+concentration from 0 to 15μmol/L,the CL intensity of the system was enhanced by one order of magnitude,and the sensitization of Ru^3+was very significant.The CL reaction conditions were optimized,and the optimum concentrations for Ru^3+,Ru(phen)2+3,Ce(Ⅳ)and H 2SO 4 were 15μmol/L,0.10 mmol/L,0.20 mmol/L and 15 mmol/L,respectively.Under the optimum conditions,the calibration curve was linear in the range of 5.0×10-9-2.0×10-6 mol/L with a detection limit of 3.8×10-9 mol/L.The proposed method had a wider linear range and lower detection limit than all other reported CL methods.The method was applied in the analysis of furosemide in furosemide tablets and furosemide injection with satisfactory results.Referring to the investigation of UV-spectra,the possible mechanism for CL reaction was proposed in this paper.

关 键 词：呋塞米 Ru^3+ 三(1 10-菲咯啉)钌(Ⅱ) 化学发光 

分 类 号：O657.3[理学—分析化学] R914.1[理学—化学]