基于CdSe@ZnS量子点水溶性纳米粒子的制备及荧光性能  

Synthesis and Fluorescence Properties Research of Water-soluble Nanoparticles Based on CdSe@ZnS Quantum Dots

作　　者：庄庆一 由芳田[1] 彭洪尚[2] ZHUANG Qing-yi;YOU Fang-tian;PENG hong-shang(Key Laboratory of Luminescence and Optical Information,Ministry of Education, Institute of Optoelectronic Technology,Beijing Jiaotong University,Beijing 100044,China;School of Science,Minzu University of China,Beijing 100081,China)

机构地区：[1]北京交通大学光电子技术研究所发光与光信息教育部重点实验室,北京100044 [2]中央民族大学理学院,北京100081

出　　处：《发光学报》2018年第10期1339-1346,共8页Chinese Journal of Luminescence

基　　金：国家自然科学基金(61775245)资助项目~~

摘　　要：作为一种新型的荧光探针,量子点(QD)已经受到越来越多的重视,制备工艺也显得格外重要。水相中合成的量子点效率低,油相中的量子点经过转相以后效率也大大衰减。本论文利用再沉淀包覆的方法制备了具有良好的水溶性的掺杂绿光Cd Se@Zn S的纳米颗粒G-NPs(534 nm)和掺杂红光Cd Se@Zn S的纳米颗粒R-NPs(610 nm),具有窄的半峰宽(G-NPs～29 nm,R-NPs～31 nm),较小的粒径(45 nm),并在此基础上通过发射光谱与荧光衰减研究了量子点之间的能量传递现象。该方法保留了量子点原来的性质,基于其优良的光学性质,对人类肝细胞肝癌细胞株(Hep G2)进行了荧光标记,从共聚焦成像实验结果看出,纳米颗粒得到了良好的吞噬效果。As a new kind of fluorescent probe,quantum dots(QD)have got more and more attention especially the preparation.However,QDs synthesized in aqueous phase suffer from the low efficiency and quantum yield of oil-soluble QDs dropped significantly after the ligand exchange.In this work,we report a type of green CdSe@ZnS-based(G-NPs,534 nm)and red CdSe@ZnS-based(R-NPs,610 nm)nanoparticles prepared via a facile reprecipitation-encapsulation method.They possess a narrow half peak width(G-NPs^29 nm,R-NPs^31 nm),smaller particle size(45 nm).Also,we discuss critical energy transfer mechanism through the emission spectrum and the fluorescence decay.This method preserves the properties of QDs,and we used the NPs to label cancer cells(HepG2)due to their excellent optical properties.Fluorescence images of the cells clearly indicate that a good fraction of the NPs has been swallowed.

关 键 词：CdSe@ZnS量子点 生物兼容性 荧光标记 荧光寿命 能量传递 

分 类 号：O482.31[理学—固体物理]