近红外到近红外Mn^(2+)掺杂NaYF_4∶Yb^(3+)/Tm^(3+)纳米粒子的制备及其生物成像  

Synthesis of NIR-to-NIR Mn^(2+) Doped-NaYF_4∶Yb^(3+)/Tm^(3+) Nanoparticles and Their Applications in Bioimaging

作　　者：罗阳[1] 廖正芳 张伟[1] 左芳[1] LUO Yang;LIAO Zheng-fang;ZHANG Wei;ZUO Fang(School of Chemistry and Environmental Protection Engineering,Southwest University for Nationalities,Chengdu 610041,China)

机构地区：[1]西南民族大学化学与环境保护工程学院,四川成都610041

出　　处：《发光学报》2018年第10期1371-1377,共7页Chinese Journal of Luminescence

基　　金：国家自然科学基金(51273220);中央高校基础科研项目(2017NZYQN17)资助~~

摘　　要：在反应温度为200℃、反应时间为8 h的温和条件下,采用水热法合成了近红外到近红外的Mn^(2+)掺杂Na YF_4∶Yb^(3+)/Tm^(3+)上转换荧光纳米粒子,再以两亲性聚合物C_(18)PMH-mPEG作为亲水性配体修饰到上转换荧光纳米粒子表面,得到具有水溶性的上转换荧光纳米粒子。然后在980 nm近红外光源激发下,测量了上转换荧光纳米粒子的荧光发射光谱,在(800±10)nm附近,观察到了较强的单近红外光发射(~3H_4→~3H_6)。对样品进行细胞毒性实验,结果表明制得的水溶性Mn^(2+)掺杂Na YF_4∶Yb^(3+)/Tm^(3+)纳米粒子具有良好的生物相容性。并进一步在小鼠体内进行了近红外成像,表明其在生物成像领域将会具有一定的应用前景。Mn 2+doped-NaYF 4∶Yb^3+/Tm^3+upconversion fluorescence nanoparticles(NPs)were synthesized by one-step hydrothermal method under 200℃for 8 h,and then the hydrophobic nanoparticles were transferred into water through coating amphiphilic polymer C 18 PMH-mPEG.Upconversion fluorescence emission spectrum of Mn 2+doped-NaYF 4∶Yb^3+/Tm^3+NPs was measured under excitation by a near-infrared(NIR)light at 980 nm.The results of cytotoxicity test show that the water-soluble Mn 2+doped-NaYF 4∶Yb^3+/Tm^3+NPs have good biocompatibility.Moreover,the prepared Mn 2+doped-NaYF 4∶Yb^3+/Tm^3+NPs are used for in mice NIR imaging,which shows that it will have a certain application prospect in bioimaging.

关 键 词：水热法 Yb^3+/Tm^3+ 上转换 纳米粒子 生物成像 

分 类 号：O482.31[理学—固体物理]