初探Ste-20激酶在调控孢子丝菌双相生长过程中的作用  

Preliminary study on the role of Ste-20 kinase in regulating biphasic growth of Sporothrix schenckii

作　　者：张振颖 胡媛媛 陈凯丽 侯彬彬[3] ZHANG Zhen-ying;HU Yuan-yuan;CHEN Kai-li;HOU Bin-bin(Department of Dermatology,University of Hong Kong-Shenzhen Hospital,Shenzhen 518053;Liaoning University,Shenyang 110031;Department of Dermatology,the Second Hospital of Dalian Medical University,Dalian 116023)

机构地区：[1]香港大学深圳医院皮肤科,深圳518053 [2]辽宁大学生命科学院,沈阳110031 [3]大连医科大学附属第一医院皮肤科,大连116023

出　　处：《中国真菌学杂志》2018年第5期268-271,261,共5页Chinese Journal of Mycology

基　　金：国家自然科学基金青年基金(81502737);国家自然科学基金面上项目(81472891)

摘　　要：目的初步研究Ste-20激酶在调控孢子丝菌双相转换过程中的作用。方法采用RNA干扰技术构建Ste-20基因沉默株,通过不同温度下的培养,观察、分析Ste-20基因干扰后对孢子丝菌在双相转换过程的影响,进一步分析Ste-20激酶在孢子丝菌中调控双相转换过程中的作用。结果 Ste-20干扰后,与标准株相比干扰菌株生长速度明显下降,往酵母相转变时细胞壁变得不完整,形态也发生变化。结论 Ste-20激酶在调控孢子丝菌双相生长过程中起一定的作用。Objective The role of Ste-20 kinase in regulating the biphasic transformation of spores was preliminarily studied.Methods Using RNA interference,observeand analysis of Ste-20 gene interference effect on sporotrichosis in dual phase transformation process,cultured under different temperatures,thus further analysis of Ste-20 kinase regulation in dual phase conversion process in the role of sporotrichosis.Results After Ste-20 interference,the growth rate significant decreased compared with interfered strain,the cell wall becomes incomplete when the yeast phase changes and morphology of the strains were changed in comparison with the standard strain.Conclusion Ste-20 kinase plays a certain role in regulating the biphasic growth of Sporothrix schenckii.

关 键 词：申克孢子丝菌 Ste-20 双相真菌 

分 类 号：R519.8[医药卫生—内科学]