离体黑果枸杞再生途径的研究  

Regeneration pathways of Lycium ruthenicum Murr in vitro

作　　者：曹君迈[1] 马海军[1] 谭亚萍 CAO Jun-mai;MA Hai-jun;TAN Ya-ping(College of Biological Sciences and Engineering,Beifang University of Nationalities,Yinchuan,Ningxia 750021,China)

机构地区：[1]北方民族大学生物科学与工程学院,宁夏银川750021

出　　处：《干旱地区农业研究》2018年第5期54-58,共5页Agricultural Research in the Arid Areas

基　　金：宁夏葡萄与葡萄酒技术创新中心2017年项目:宁夏葡萄与枸杞果实生理特性研究;国家自然科学基金(31360468)

摘　　要：以黑果枸杞的叶片和茎段为外植体,采用MS为基本培养基,研究激素对经历愈伤组织诱导、丛生芽诱导以及叶片直接诱导小植株再生途径的影响。实验结果表明,诱导茎段、叶片形成愈伤组织的适宜培养基分别为MS+2,4-D0.3 mg·L^(-1)、MS+2,4-D0.4 mg·L^(-1),其诱导率均为100%;诱导茎段、愈伤组织分化形成丛生芽的适宜培养基分别为MS+6-BA0.2 mg·L^(-1)+KT0.1 mg·L^(-1)、MS+6-BA0.5 mg·L^(-1),而愈伤组织分化出的丛生芽均发生玻璃化现象,其增殖系数分别为32.3倍、47.1倍;诱导叶片分化形成植株的适宜培养基为MS+NAA0.01 mg·L^(-1),其再生植株诱导率为33.3%。结论:黑果枸杞再生能力强,以上途径均能形成再生植株,其最佳的离体繁殖途径为茎段诱导丛生芽形成再生植株。Effects of hormones on the regeneration pathway of callus-induced plantlets,clump shoot-induced plantlets and leaf\\induced plantlets were investigated by explants,leaves and stem segments of Lycium ruthenicum Murr and MS basic medium.The results showed that the appropriate medium for callus induction from stem segments and leaves was MS+2,4-D 0.3 mg·L-1 and MS+2,4-D 0.4 mg·L-1,respectively and both of the induction rate were 100%.The appropriate medium for differentiation of stem segments and calli into clump shoot was MS+6-BA 0.2 mg·L-1+KT 0.1 mg·L-1 and MS+6-BA 0.5 mg·L-1,respectively,with multiplication coefficient of 32.3 times and 47.1 times,respectively.However,vitrification occurred in clump shoot differentiated from calli.The appropriate medium for inducing leaves to differentiate into plants was MS+NAA 0.01 mg·L-1,with induction rate of regenerated plants of 33.3%.The regeneration ability of Lycium ruthenicum Murr was strong,and regenerated plants could be formed through above pathways.The optimal in vitro propagation pathway was stem segments inducing clump shoot to form regenerated plants.

关 键 词：黑果枸杞 外植体 愈伤组织 丛生芽 再生植株 

分 类 号：S567.19[农业科学—中草药栽培]