皮肤反射式共聚焦显微成像扫描畸变校正  

Correction of reflectance confocal microscopy for skin imaging distortion due to scan

作　　者：刘创 张运海(指导)[1] 黄维[1,2] 唐玉国 Liu Chuang;Zhang Yunhai;Huang Wei;Tang Yuguo(Jiangsu Key Laboratory of Medical Optics,Suzhou Institute of Biomedical Engineering and Technology,Chinese Academy of Sciences,Suzhou 215163,China;University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]中国科学院苏州生物医学工程技术研究所,江苏省医用光学重点实验室,江苏苏州215163 [2]中国科学院大学,北京100049

出　　处：《红外与激光工程》2018年第10期147-152,共6页Infrared and Laser Engineering

基　　金：国家重点研发计划(2017YFC0110303)；国家重大科研装备研制项目(ZDYZ2013-1)；江苏省基础研究计划青年基金(BK20160363)；苏州应用基础研究计划(SYG201510)；苏州市民生科技项目(SS201643)。

摘　　要：皮肤反射式共聚焦显微镜是一种重要的皮肤影像学诊断工具,其采用共振型振镜扫描成像会带来非线性畸变,为校正这种畸变,提出一种像素值反正弦过采样信号重建的RCM图像畸变校正方法,对间距为20μm的矩形光栅扫描成像实验表明,在校正畸变前光栅图像间距的标准差为7.78μm,图像的畸变率达到38.9%,经过像素值反正弦过采样信号重建后,光栅图像间距的标准差缩小为0.85μm,畸变率缩小到4.2%。结合分辨率板和实际人皮肤成像情况,表明文中提出的畸变校正方法能够较好地校正共振型振镜引起的图像畸变,满足人皮肤实时无创影像学诊断要求。Reflectance Confocal Microscopy for skin(RCM)is an important skin imaging diagnostic tool,which uses resonant galvanometer that will result in nonlinear distortion at image.In order to correct the distortion,a method of anti-sine signal oversampling based pixel using isochronous sampling system to correct the distorted RCM image caused by resonant galvanometer was presented.The results of experiments on the rectangular grating with a pitch of 20μm indicate that,the standard deviation of the grating spacing is 7.78μm.The distortion rate of image before being corrected is 38.9%.The standard deviation of the image corrected by the anti-sine signal over-sampling based pixel method is 0.85μm,so the distortion rate is reduced to 4.2%.According to the results of a resolution plate and the actual human skin imaging,the distortion correction method proposed in this paper can correct the image distortion caused by resonant galvanometer and meet the human skin real-time non-invasive imaging diagnostic requirements.

关 键 词：共聚焦显微镜 共振型振镜 非线性畸变 

分 类 号：TP391.4[自动化与计算机技术—计算机应用技术]