菲律宾蛤仔橙蛤、斑马蛤和白蛤群体酚氧化酶活性的比较研究  

A comparison of phenoloxidase activity in the orange-,zebra-and white-groups of Manila clam Ruditapes philippinarum

作　　者：丁鉴锋 温嫱 霍忠明 聂鸿涛 闫喜武 DING Jian-feng;WEN Qiang;HUO Zhong-ming;NIE Hong-tao;YAN Xi-wu(College of Fisheries and Life Science,Dalian Ocean University,Dalian 116023,China;Engineering Research Center of Shellfish Culture and Breeding in Liaoning Province,Dalian 116023,China)

机构地区：[1]大连海洋大学水产与生命学院,辽宁大连116023 [2]辽宁省贝类良种繁育工程技术研究中心,辽宁大连116023

出　　处：《大连海洋大学学报》2018年第5期558-563,共6页Journal of Dalian Ocean University

基　　金：辽宁省教育厅科研项目(L2014276);国家"863"计划项目(2012AA10A410-2)

摘　　要：为研究酚氧化酶(PO)在贝类体色形成和非特异性免疫过程中的重要作用,检测了壳长为(2. 1±0. 3) cm的3种壳色菲律宾蛤仔Ruditapes philippinarum (橙蛤、斑马蛤和白蛤)血淋巴细胞和外套膜中的酚氧化酶活性,并对用脂多糖(LPS)和肽聚糖(PGN)刺激后其酶活性的变化进行了测定。结果表明:3种壳色蛤仔血淋巴细胞中酚氧化酶活性均显著高于外套膜中的酶活性(P<0. 05);注射脂多糖后,橙蛤、斑马蛤和白蛤血淋巴细胞中酚氧化酶活性分别在3、12、12 h达到最大值,斑马蛤血淋巴细胞中酚氧化酶活性在12 h显著高于橙蛤(P<0. 05),橙蛤、斑马蛤和白蛤外套膜中酚氧化酶活性分别在刺激后6、6、24h时达到最大值;注射肽聚糖后,橙蛤血淋巴细胞中酚氧化酶活性在24 h达到最低值,斑马蛤和白蛤血淋巴细胞中的酚氧化酶活性分别在刺激后12 h和6 h达到最大值,白蛤血淋巴细胞中酚氧化酶活性在注射后6 h显著高于橙蛤和斑马蛤(P<0. 05),斑马蛤血淋巴细胞中酚氧化酶活性在刺激后12 h显著高于橙蛤和白蛤(P<0. 05),橙蛤外套膜组织中的酚氧化酶活性在注射肽聚糖后12 h达到最大值;不同壳色蛤仔体内酚氧化酶对细菌提取物刺激所表现出的不同反应模式,证实了蛤仔壳色形成过程与免疫能力可能具有重要的关联。本研究结果可为菲律宾蛤仔的壳色选育提供理论支持。Phenoloxidase(PO)activity was determined in hemocytes and mantle tissues of Manila clam Ruditapes philippinarum with three shell colors(orange,zebra and white)with shell length of(2.1±0.3)cm,and the hemolymph phenoloxidase activity were also measured in the Manila clam with injection of lipopolysaccharide(LPS)at a dose of 100μg/mL and peptidoglycan(PGN)at a dose of 20μg/mL 0,3,6,12,24,and 48 h after the administration.The results showed that there was significantly higher PO activity in hemocytes than that in mantle in all the three shell-color groups(P<0.05),the maximal level of PO activity in hemocytes of the clam injected with LPS at 3 h in orange,and 12 h in zebra and white clam groups,significant higher in zebra group than that in orange group at 12 h(P<0.05).In the mantle,however,the maximal level of PO activity was observed at 6 h in orange and zebra shell groups and 24 h in white shell color group.The PO activity in hemocytes was found to be declined in orange shell-color clam injected with PGN,the minimal value at 24 h,while the PO activity in hemocytes was clam enhanced in zebra and white color groups,with the maximal level at 12 h in zebra clam and 6 h in white color clam,and significant higher in mantle of zebra color group than that in orange and white color groups(P<0.05).The maximal PO activity in the mantle was detected in the orange color group clam at 12 h after PGN injection,indicating that the responds of PO activity to pathogens are varied with clams with different shell colors,which is involved in close correlation between shell pigmentation and immunity in Manila clam.These findings will provide theoretical support for shell color selection of Manila clam.

关 键 词：菲律宾蛤仔 壳色 血淋巴细胞 外套膜 酚氧化酶 

分 类 号：S968.31[农业科学—水产养殖]