低盐胁迫对白条双锯鱼相关生理指标的影响  

Effects of low salinity stress on physiological indices in tomato clownfish Amphiprion frenatus

作　　者：李晓雨 田燚 王伟 李延涛 刘钢 郭然[1] 丛佳 LI Xiao-yu;TIAN Yi;WANG Wei;LI Yan-tao;LIU Gang;GUO Ran;CONG Jia(College of Fisheries and Life Science,Dalian Ocean University,Dalian 116023,China;Key Laboratory of Mariculture&Stock Enhancement in North China s Sea,Dalian Ocean University,Dalian 116023,China;Dalian Shengya Tourism Holding Company Limited,Dalian 116023,China;Key Laboratory of Hydrobiology in Liaoning Province,Dalian Ocean University,Dalian 116023,China)

机构地区：[1]大连海洋大学水产与生命学院,辽宁大连116023 [2]农业部北方海水增养殖重点实验室,辽宁大连116023 [3]大连圣亚旅游控股股份有限公司,辽宁大连116023 [4]大连海洋大学水生生物学实验室,辽宁大连116023

出　　处：《大连海洋大学学报》2018年第5期614-619,共6页Journal of Dalian Ocean University

基　　金：辽宁省百千万人才支持计划项目(2014921067);大连市科技之星计划项目(2015R081)

摘　　要：为研究低盐胁迫对白条双锯鱼Amphiprion frenatus生理指标的影响,试验设置3个盐度组(盐度20、25、31),在水温(25. 0±0. 5)℃条件下,低盐胁迫后3、12、48 h时测定湿体质量为(5. 42±1. 29) g的成体白条双锯鱼的血细胞总数、鳃丝Na^+-K^+-ATP酶(NKA)活力、肝脏超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活力的变化。结果表明:整个低盐胁迫过程中,各试验组白条双锯鱼个体活力正常,无死亡现象;盐度胁迫后48 h时,鱼体血细胞总数随盐度的降低而减少,且20盐度组在48 h时鱼体血细胞数量显著低于对照组(盐度31)和25盐度组(P<0. 05);鳃丝Na^+-K^+-ATP酶(NKA)活力在盐度20、25胁迫下随时间的延长呈先升高后下降的趋势,NKA活力在盐度25整个胁迫期间均显著高于对照组和20盐度组(P<0. 05),在盐度20胁迫下,仅在12 h时显著高于对照组(P<0. 05); 20、25盐度组肝脏SOD活性在各时间点均高于对照组,但无显著性差异(P>0. 05);整个试验期间内,20盐度组CAT活力3 h时显著高于对照组(P<0. 05),而12 h和48 h均显著低于对照组(P<0. 05),25盐度组CAT活力与对照组无显著性差异(P>0. 05)。研究表明,盐度20、25胁迫48 h内会显著影响白条双锯鱼的血细胞数、Na^+-K^+-ATP酶活力,盐度20会显著影响该鱼的CAT活力,但整个低盐胁迫过程并未影响鱼体的摄食和存活,相关生理指标的变化可为白条双锯鱼的淡化养殖提供参考依据。The activities of liver superoxide dismutase(SOD)and catalase(CAT),total count of blood cells,and gill Na+-K+-ATPase activity were measured in adult tomato clownfish Amphiprion frenatus with body weight of(5.42±1.29)g exposed to a salinity of 31,25 and 20 for 3 h,12 h and 48 h to study the effect of low salinity stress on the physiological indices of the tomato clownfish.No dead tomato clownfish were observed in three experimental groups during the low salinity stress.However,the total number of blood cells was found to be decreased with decreasing salinity for 48 h salinity stress,significantly lower at salinity of 20 in 48 h exposure than that in the other two groups(P<0.05).The activity of Na+-K+-ATPase(NKA)was increased firstly and then decreased with the change in salinity under a salinity of 20 and 25,significantly higher a salinity of 25 than those in the control group and in the 20 salinity group during the whole stress period(P<0.05).In liver SOD activities were higher in the fish exposed to salinity of 20 and 25 than that in the control group.During the whole experiment,the CAT activity in salinity of 20 was significantly different from that in the control group(P<0.05);significantly lower in the salinity of 25 than that in the control group only in 48 h(P<0.05).The findings indicate that lower salinity(20 and 25)for 48 h will affect count of blood cells,and activities of Na+-K+-ATPase and CAT instead of survival and growth of tomato clownfish during the experiment stage,providing basis with the desalination culture of tomato clownfish.

关 键 词：白条双锯鱼 低盐胁迫 血细胞数 Na+-K+-ATP酶活性 SOD活力 CAT活力 

分 类 号：S965.82[农业科学—水产养殖]