人参水解物中人参二醇的工艺优化及其HPLC-ELSD含量测定方法  

Process Optimization of Panaxadiol in Ginseng Hydrolysate and Its Content Determination by HPLC-ELSD

作　　者：刘小妹 施崇精 程中琴 王姗姗 袁强华[2] 宋英[2] LIU Xiao-mei;SHI Chong-jing;CHENG Zhong-qin;WANG Shan-shan;YUAN Qiang-hua;SONG Ying(Pharmacy College,Chengdu University of TCM,Chengdu 610075,China;The Affiliated Hospital of Chengdu University of TCM,Chengdu 610072,China)

机构地区：[1]成都中医药大学药学院,成都610075 [2]成都中医药大学附属医院,成都610072

出　　处：《天然产物研究与开发》2018年第10期1752-1757,1781,共7页Natural Product Research and Development

基　　金：国家中医药管理局国家中药标准化项目(ZYBZH-Y-SC-41);四川省中医药管理局;其他中医药支出(210069)

摘　　要：采用HPLC-ELSD建立人参水解物中人参二醇的含量测定方法,并探讨人参二醇的最佳水解条件。HPLC-ELSD具体条件为:Agilent ZORBAX SB-C18柱(4. 6 mm×150 mm,5μm),流动相为乙腈-水(75∶25),混合方式:离线混合,流速0. 8 mL/min,柱温30℃,检测器参数为漂移管温度85℃,蒸发器气体流速:2. 6 mL/min。以人参二醇含量为指标,从盐酸用量、水解温度、水解时间3个方面优化水解工艺。根据回归方程,人参二醇在0. 494～1. 978μg(R2=0. 9998)时线性关系良好。回收率在98. 79%～101. 37%,RSD为1. 07%(n=6)。在此测定方法下优选出人参二醇的最佳水解条件为:酸的浓度控制在5%、水解温度80℃、水解3 h。本研究首次建立了HPLC-ELSD测定人参中人参二醇含量的方法,该法准确可靠、方便简单,优选的工艺为人参二醇的综合开发利用提供科学依据。The determination method of panaxadiol in Ginseng hydrolysate was established by HPLC-ELSD.The optimal hydrolysis condition of panaxadiol was investigated.For HPLC-ELSD,an Agilent ZORBAX SB-C 18 column(4.6 mm×150 mm,5μm)was applied,the mobile phase consisted of acetonitrile-water(75∶25),Hybrid method:offline hybrid,the flow rate was 0.8 mL/min,the column temperature was 30℃,the drift tube temperature of ELSD was set at 85℃,and the flow rate of air was 2.6 mL/min.Based on panaxadiol content,the hydrolysis process was optimized from three aspects:the amount of hydrochloric acid,the hydrolysis temperature and the hydrolysis time.According to the regression equation,it was found that as for panaxadiol,the linear detection range was 0.494-1.978μg(R 2=0.9998),the recovery was from 98.79%-101.37%,and the relative standard deviation was 1.07%(n=6).Under the determination method,the optimal hydrolysis conditions were:acid concentration control at 5%,hydrolysis temperature 80℃,and time was 3 h.In conclusion,a HPLC-ELSD method for determination of panaxadiol in Ginseng was established for the first time,the method was reliable and easy.The optimized process provided scientific basis for the comprehensive exploitation and utilization of panaxadiol.

关 键 词：人参 人参二醇 水解 高效液相色谱-蒸发光散射检测器法 

分 类 号：R93[医药卫生—生药学]