D-柠檬烯对衣霉素诱导的胰腺MIN6细胞损伤的保护作用  

D-limonene Protects Pancreatic MIN6 Cells against Tunicamycin Induced Cellular Damage

作　　者：付柏婷 张萍[1] FU Bai-ting;ZHANG Ping(Key Laboratory of Tropical Plant Resource Sciences and Sustainable Use,Xishuangbanna Tropical Botanical Garden,Chinese Academy of Sciences,Xishuangbanna 666303,China;University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]中国科学院西双版纳热带植物园热带植物资源可持续利用重点实验室,西双版纳666303 [2]中国科学院大学,北京100049

出　　处：《天然产物研究与开发》2018年第10期1793-1797,1804,共6页Natural Product Research and Development

基　　金：中国科学院战略生物资源服务网络计划活性天然化合物发现;评价与转化项目(Y7ZK201B01)

摘　　要：本研究主要探讨D-柠檬烯对衣霉素(Tm)诱导胰腺MIN6细胞损伤的保护作用。设置溶剂对照组、Tm组、4-苯基丁酸(PBA)组和不同浓度的D-柠檬烯组,用Griess试剂检测Tm刺激胰岛β细胞产生的NO水平;Annexin V-FITC/PI双染法流式检测细胞凋亡; RT-PCR法检测mRNA的表达水平; Western Blot法检测相关蛋白的表达。结果显示,D-柠檬烯组与Tm组相比,NO产生量减少、细胞凋亡率降低、内质网应激相关基因sXbp1和CHOP的mRNA表达量显著降低、p-eIF2α的蛋白表达量显著下降。结果表明D-柠檬烯对衣霉素(Tm)诱导的胰腺MIN6细胞损伤具有显著的保护作用。In this study,the protective effect of D-limonene on the cellular damage induced by tunicamycin in pancreatic MIN6 cells was investigated.Cells were pretreated with vehicle,PBA and different doses of D-limonene,and then cultured with Tm for 5-24 h.NO production was detected by Griess reagent method.Cells were stained with Annexin V-FITC/PI for apoptosis by flow cytometry.RT-PCR was used to analyze mRNA expression of ER stress related genes sXbp1 and CHOP.Western Blot was used to detect eIF2αprotein expression.Our results showed that,compared with Tm-treated cells,cells treated with D-limonene exhibited significantly decreased NO production and apoptosis.Furthermore,D-limonene treatment resulted in significantly lower mRNA levers of sXbp1 and CHOP as well as lower level of protein expression of phosphorylated eIF2α.These results demonstrated that D-limonene had a significant protective effect on cellular damage induced by tunicamycin in pancreatic MIN6 cells.

关 键 词：D-柠檬烯 MIN6 内质网应激 凋亡 细胞损伤 

分 类 号：R967[医药卫生—药理学]