外源La(Ⅲ)和Si对水稻幼苗植硅体固镧能力的影响  

Effect of exogenous lanthanum and silicon on the biosequestration ability of lanthanum by phytoliths in rice seedlings

作　　者：司勇 王丽红[1,2] 周青[1,2,4] SI Yong;WANG Li-hong;HOU Qing(School of Environment and Civil Engineering,Jiangnan University,Wuxi 214122,China;State Key Laboratory of Food Science and Technology,Jiangnan University,Wuxi 214122,China;College of Resources Environment and Tourism Management,West Anhui Univer.sity,Lu′an 237012,China;Jiangsu Cooperative Innovation Center of Technology and Materials for Water Treatment,Suzhou University of Science and Technology,Suzhou 215009,China)

机构地区：[1]江南大学环境与土木工程学院,江苏无锡214122 [2]江南大学食品科学与技术国家重点实验室,江苏无锡214122 [3]皖西学院环境与旅游学院,安徽六安237012 [4]苏州科技大学江苏省水处理技术和材料合作创新中心,江苏苏州215009

出　　处：《农业环境科学学报》2018年第10期2101-2109,共9页Journal of Agro-Environment Science

基　　金：江苏省普通高校科研创新计划项目(KYLX15_1164);国家自然科学基金项目(31170477;21371100;21501068)~~

摘　　要：为研究环境中稀土元素(REEs)和硅(Si)对植物植硅体固镧能力(PLSA)的影响,以水稻(Oryza sativa L.)为代表,通过设置不同浓度镧[La(Ⅲ)]和Si正交实验,利用微波消解法提取根、茎和叶植硅体,以电感耦合等离子体原子发射光谱和实时荧光定量PCR等实验手段揭示环境La(Ⅲ)对水稻各器官PLSA的影响。结果显示,低浓度La(Ⅲ)使叶哑铃型植硅体大小和根Lsi1表达增加,促进叶和根PLSA,而高浓度La(Ⅲ)会减少叶哑铃型植硅体大小,降低根Lsi1和Lsi2表达,抑制根、茎和叶PLSA。外源La(Ⅲ)是提高根、茎和叶植硅体镧固定效率的重要因素。与单一La(Ⅲ)浓度影响相比,La(Ⅲ)与Si的复合处理显著促进叶和茎PLSA,外源Si能够缓解高浓度La(Ⅲ)对根、茎和叶PLSA的抑制作用。研究证明不同浓度的外源La(Ⅲ)和Si能够改变水稻不同器官的PLSA。Rare earth elements(REEs)in the environment enter plant tissues through the plant′s roots,after which they can be sequestrated by phytoliths in plants.Silicon(Si)can affect the formation of plant phytoliths.Currently,the effects of REEs and Si on the sequestration ability of lanthanum[La(Ⅲ)]by the phytoliths(PLSA)in plants are not yet fully understood.In this study,rice(Oryza sativa L.)was selected as a representative of plants,and orthogonal experiments were set up under various concentrations of La(Ⅲ)and Si.Phytoliths were extracted from different parts of rice seedlings(roots,stems,and leaves)via the microwave digestion method,and the effects of exogenous La(Ⅲ)on the PLSA of different rice organs were revealed by using inductively coupled plasma atomic emission spectrometry and quantitative real-time PCR.The results showed that a low concentration of La(Ⅲ)increased the size of dumbbell phytoliths in leaves and the expression level of Lsi1 in roots and promoted the PLSA in roots and leaves.Meanwhile,a high concentration of La(Ⅲ)decreased the size of dumbbell phytoliths in leaves and the expression levels of Lsi1 and Lsi2 in roots and inhibited the PLSA in roots,stems,and leaves.Exogenous La(Ⅲ)was an important factor for promoting the sequestration efficiency of La(Ⅲ)by phytoliths in leaves,stems,and roots.Compared to the single treatment with La(Ⅲ),the combined treatment with La(Ⅲ)and Si promoted the PLSA in leaves and stems significantly and exogenous Si alleviated the inhibitory effects of a high concentration of La(Ⅲ)on the PLSA of leaves,roots,and stems.This study demonstrated that different concentrations of exogenous La(Ⅲ)and Si could change the PLSA in different organs of rice seedlings.

关 键 词：水稻 镧 硅 植硅体 植硅体镧 

分 类 号：X503.231[环境科学与工程—环境工程]