Zileuton对血管紧张素Ⅱ诱导心肌细胞肥大的影响研究  

Effect of zileuton on cardiomyocytes hypertrophy induced by angiotensinⅡ

作　　者：谢赛阳 罗梅[3] 吴青青[1,2] 魏丽[2,4] 陈娇娇[2,4] 邓伟 XIE Saiyang;LUO Mei;WU Qingqing;WEI Li;CHEN Jiaojiao;DENG Wei(Department of Cardiology,Renmin Hospital of Wuhan University,Wuhan 430060,China;Cardiovascular Research Institute of Wuhan University,Wuhan 430060,China;Department of Cardiology,The Fifth Affiliated Hospital,Xinjiang Medical University,Urumqi 830011,China;Department of Pediatrics,Renmin Hospital of Wuhan University,Wuhan 430060,China)

机构地区：[1]武汉大学人民医院心内科,武汉430060 [2]武汉大学,心血管病研究所,武汉430060 [3]新疆医科大学第五附属医院心内科,乌鲁木齐830011 [4]武汉大学人民医院儿科,武汉430060

出　　处：《新疆医科大学学报》2018年第10期1256-1259,共4页Journal of Xinjiang Medical University

基　　金：国家自然科学基金(81470516,81530012)

摘　　要：目的探讨5-脂氧合酶特异性抑制剂Zileuton对血管紧张素Ⅱ(AngⅡ)诱导新生大鼠心肌细胞肥大的影响。方法使用不同浓度Zileuton(1、10、50、100μM)和AngⅡ(1μM)共同孵育新生大鼠心肌细胞12h后,采用α-actin荧光染色评估单个心肌细胞肥大程度,Real-time PCR方法检测心肌细胞肥大标志物心房利钠肽(atrial natriuretic peptide,ANP)、B型脑钠肽(B-type natriuretic peptide,BNP)、心肌球蛋白重链(β-myosinheavy chain,β-MHC)的mRNA表达变化。选择50μM浓度的Zileuton与AngⅡ(1μM)共同孵育新生大鼠心肌细胞6、12、24h,观察Zileuton对AngⅡ诱导新生大鼠心肌细胞肥大抑制作用的时间相关性。结果 Zileuton能够浓度依赖性的抑制AngⅡ诱导的新生大鼠心肌细胞肥大,同时缓解AngⅡ诱导的心肌细胞肥大标志物ANP、BNP、β-MHC的mRNA表达增加。结论 Zileuton能够抑制AngⅡ诱导的心肌细胞肥大,5-脂氧合酶可能成为抑制心肌重构、治疗心力衰竭的潜在靶点。Objective To investigate the effect of zileuton(5-lipoxygenase inhibitor)on cardiomyocytes hypertrophy induced by AngiotensinⅡ(AngⅡ).Methods The neonatal rat cardiomyocytes were co-incubated with different concentrations of zileuton(1,10,50 and 100μM)and AngⅡ(1μM)for 12 h.The cells were characterized by immuncyto-chemistry ofα-actin to examine increasing cell surface area and real-time PCR was used to measure mRNA levels of hypertrophic markers of ANP,BNP andβ-MHC to clear the effect of zileuton on neonatal rat cardiomyocytes hypertrophy.Results It was found neonatal rat cardiomyocytes treated with zileuton could be protected from AngⅡ-induced hypertrophy.The increasing of mRNA levels of ANP,BNP andβ-MHC in AngⅡ-stimulated cells were also down-regulated by zileuton treatment.Conclusion Zileuton attenuates cardiomyocytes hypotrophy which induced by AngⅡ.

关 键 词：5-脂氧合酶 ZILEUTON 血管紧张素Ⅱ 心肌细胞 

分 类 号：R5[医药卫生—内科学]