基于脱氧核酶的铅离子生物传感器研究进展  

Advances in DNAzyme-based Pb^(2+)-specific biosensors

作　　者：杨荣[1] 宋丹[1] 周小红[2] 龙辉 龙峰[1] YANG Rong;SONG Dan;ZHOU Xiaohong;LONG Hui;LONG Feng(School of Environment and Natural Resources,Renmin University of China,Beijing 100872;School of Environment,Tsinghua University,Beijing 100084;Institute of Product Quality Inspection of Guangxi Zhuang Autonomous Region,Nanning Guangxi 530007)

机构地区：[1]中国人民大学环境学院,北京100872 [2]清华大学环境学院,北京100084 [3]广西壮族自治区产品质量检验研究院,广西南宁530007

出　　处：《环境污染与防治》2018年第10期1175-1180,共6页Environmental Pollution & Control

基　　金：国家自然科学基金资助项目(No.21077063;No.21277173);国家重大仪器专项第五课题(No.2012YQ3011105)

摘　　要：铅离子(Pb^(2+))是一种毒性强、危害大的重金属污染物,对其进行及时准确地检测意义重大。对各类环境样品中的Pb^(2+)进行检测的方法很多,其中基于脱氧核酶设计的生物传感器是一种灵敏度高、选择性好的方法。对脱氧核酶进行了介绍,总结了脱氧核酶在Pb^(2+)生物传感器设计中的应用,重点综述了基于RNA剪切型和G-四链体型两种脱氧核酶设计的荧光型、比色型和电化学型Pb^(2+)生物传感器的发展历史以及研究进展,并对将来的研究方向进行了展望。Lead ion(Pb 2+)is a kind of heavy metal pollutant that is harmful to human health with high toxicity.It is extremely important to develop a fast and accurate method to detect the concentration of Pb 2+in environmental samples.Various Pb 2+detection methods have been designed.Among them,DNAzyme-based biosensors have received considerable attention because of their high sensitivity and selectivity.In this review,the DNAzyme and its applications for Pb 2+biosensor were introduced.Various fluorescent,colorimetric and electrochemical Pb 2+-specific biosensors based on RNA splicing DNAzyme and G-quadruplex DNAzyme were reviewed.The perspective of DNAzyme-based Pb 2+-specific biosensor was also discussed.

关 键 词：脱氧核酶 铅离子 G-四链体 生物传感器 

分 类 号：X830[环境科学与工程—环境工程]