牛骨髓油脂肪酸组成与活性研究  

Research on Fatty Acids Composition and Bioactivity of Bovine Bone Marrow Oil

作　　者：帕尔哈提.柔孜 阿布力米提.伊力 木合布力.阿布力孜 马尔霍夫.木合布力 克依沙尔.艾斯卡尔 赵超然 王成如 PARHAT Rozi;ABULIMITI Yili;MOURBOUL Ablise;MARGHOF Mourboul;KAISER Askar;ZHAO Chao-ran;WANG Cheng-ru(Xinjiang Technical Institute of Physics and Chemistry,Chinese Academy of Sciences,Urumqi 830011,Xinjiang,China;University of the Chinese Academy of Sciences,Beijing 100049,China;College of Pharmacy,Xinjiang Medical University,Urumqi 830011,Xinjiang,China;Xinjiang Faradin Biotechnology Limited Company,Urumqi 830011,Xinjiang,China)

机构地区：[1]中国科学院新疆理化技术研究所,新疆乌鲁木齐830011 [2]中国科学院大学,北京100049 [3]新疆医科大学药学院,新疆乌鲁木齐830011 [4]新疆法拉丁生物科技有限责任公司研发中心,新疆乌鲁木齐830011

出　　处：《食品研究与开发》2018年第21期59-66,共8页Food Research and Development

基　　金：乌鲁木齐高新技术产业开发区"十百千"高端人才引进项目基金资助[乌高(新)党办发(2017)24号]

摘　　要：以牛骨髓油为原料,分别用石油醚、正己烷、无水甲醇及无水乙醇制备不同提取物,经甲酯化后,通过气质联用仪分析脂肪酸组成,测定其抗氧化和抗菌活性以确定最佳提取溶剂。结果表明:4种溶剂提取的牛骨髓油中饱和脂肪酸(saturated fatty acid,SFA)/不饱和脂肪酸(unsaturated fatty acids,UFA)比例分别为1/1.4、1/1.41、1/1.20、1/1.25,主要脂肪酸包括油酸(46.22%～50.42%)、棕榈酸(22.20%～24.10%)、硬脂酸(13.41%～16.49%)、亚油酸(1.91%～3.75%),不饱和脂肪酸含量高于饱和脂肪酸。其中正己烷提取效果最好,不饱和脂肪酸含量达58.42%,且具有一定的抗氧化和抑菌活性。这对牛骨髓油的质量标准建立与相关产品开发利用具有指导意义和提供参考依据。The bovine bone marrow oil(BBMO)was extracted with petroleum ether,hexane,anhydrous methanol and ethanol,respectively.Following the methylation,the relative fatty acid content was analyzed with gas chromatography-mass spectrometry(GC/MS),and the best extraction solvent evaluated by antioxidant and antimicrobial test.The results indicated that proportion of BMO saturated fatty acid(SFA)rand unsaturated fatty acid(UFA)ration were 1/1.4,1/1.41,1/1.20,1/1.25,respectively.Mainly fatty acids includes oleic acid(46.22%-50.42%),palmitic acid(22.20%-24.10%),stearic acid(13.41%-16.49%)and linoleic acid(1.91%-3.75%).In above acids content of UFA higher than SFA,and the n-hexane was identified as optimum solvent,in which UFA content reached to 58.42%,meanwhile,it posses some extent of antioxidant and antimicrobial activity.This research may provide the foundation for establishing quality standards and assist the scientific development of health products contained with BBMO.

关 键 词：牛骨髓油 脂肪酸 气相色谱-质谱联用仪 抗氧化 抗菌 

分 类 号：TS251.94[轻工技术与工程—农产品加工及贮藏工程]