响应面法优化猪脑中神经节苷脂的硅胶柱层析纯化  

Optimization of Silica Gel Column Chromatography for Purification of Gangliosides from Pig Brain by Response Surface Methodology

作　　者：龚鸣敬 李雪联 古明鲜 万书林 管天冰 何平 王建 戴传云[1] GONG Ming-jing;LI Xue-lian;GU Ming-xian;WAN Shu-lin;GUAN Tian-bing;HE Ping;WANG Jian;DAI Chuan-yun(Department of Chemical and Chemical Engineering,Chongqing University of Science and Technology,Chongqing Key Laboratory of Industrial Fermentative Microbes,Chongqing 401331,China;Chongqing HUANRUI Bio-technology CO.,LTD.,Chongqing 401332,China;Chongqing Bisan National Agricultural Science and Technology Park,Chongqing 402760,China)

机构地区：[1]重庆科技学院化学化工学院工业发酵微生物重庆市重点实验室,重庆401331 [2]重庆寰瑞生物技术有限公司,重庆401332 [3]重庆璧山国家农业科技园区,重庆402760

出　　处：《食品研究与开发》2018年第21期82-86,共5页Food Research and Development

基　　金：重庆科技学院研究生科技创新计划项目(YKJCX1620507)

摘　　要：以猪脑组织提取物为原料,采用硅胶柱层析法分离纯化神经节苷脂。利用单因素试验和响应面法,确定最佳纯化工艺条件:质量浓度为7.75%的样品溶液,以2.5 BV/h流速上样至装有34.89 g硅胶的层析柱后,采用乙酸乙酯与甲醇(体积比为4∶1)的混合溶剂以2.0 BV/h流速洗脱,洗脱产物中神经节苷脂的回收率为86.9%,目标化合物含量为纯化前的2.89倍,因此该纯化工艺方便可行。The gangliosides in pig brain tissue extract was purified by method of slica column chromatography.Based on single factor experiments and response surface methodology,the optimum conditions were as follows:the sample that gangliosides concentration of crude extract was 7.75%was loaded on the column which filled with 34.89 g slica at flow rate of 2.5 BV/h,then the eluent contained ethyl acetate and methyl alcohol,which volume ratio was 4∶1,washed the colume at flow rate of 2.0 BV/h,and the gangliosides in product was analysed.The recovery of gangliosides in final product was 86.9%,and the content has increased 2.89 times from the rate before purification.Therefore,the process is convenient and feasible.

关 键 词：响应面法 柱层析 神经节苷酯 纯化 

分 类 号：TQ464[化学工程—制药化工]