重组腺病毒Ad-GFP-C197对3种肿瘤细胞的抑制作用研究  

Study on Inhibitory Effects of Recombinant Adenovirus Ad-GFP-C197 on 3 Kinds of Tumor Cells

作　　者：邬贤[1,2] 黄莹 李舒婕 段萍萍 米鹏程 陈文瑛[1,2] WU Xian;HUANG Ying;LI Shujie;DUAN Pingping;MI Pengcheng;CHEN Wenying(School of Pharmaceutical Sciences,Southern Medical University,Guangzhou 510515,China;Dept.of Pharmacy,the Third Affiliated Hospital of Southern Medical University,Guangzhou 515000,China;Dept.of Pharmacy,Shenzhen Hospital of Southern Medical University,Guangdong Shenzhen 518000,China)

机构地区：[1]南方医科大学药学院,广州510515 [2]南方医科大学第三附属医院药学部,广州515000 [3]南方医科大学深圳医院药学部,广东深圳518000

出　　处：《中国药房》2018年第20期2800-2804,共5页China Pharmacy

基　　金：广州市天河区科技计划项目(No.穗天科工信字[2016]19号-201604KW026)

摘　　要：目的:研究以腺病毒载体系统装载人端粒酶逆转录酶C端片段(C197)制成的重组腺病毒Ad-GFP-C197对3种肿瘤细胞增殖的抑制作用。方法:采用HEK293细胞扩增并纯化Ad-GFP-C197。采用Ad-GFP-C197分别感染人胃癌细胞SGC7901、人乳腺癌细胞MCF7和人结直肠癌细胞CaCO2。以空白腺病毒载体(Ad-GFP)为参比,采用Western blot法检测Ad-GFP-C197感染后3种肿瘤细胞中C197的蛋白表达水平;采用MTT法检测Ad-GFP-C197感染后对3种肿瘤细胞的抑制作用,绘制细胞增殖曲线并计算增殖抑制率。结果:Ad-GFP感染的3种肿瘤细胞中均未检测到C197蛋白表达,而Ad-GFP-C197感染的上述细胞中C197蛋白均有明显表达;Ad-GFP-C197感染后3种肿瘤细胞的增殖曲线随时间延长而呈明显抑制现象,其增殖抑制率最高可达37.31%～41.42%。结论:Ad-GFP-C197对SGC7901细胞、MCF7细胞、CaCO2细胞增殖均有明显的抑制作用,且起效迅速,能够克服其他端粒酶抑制剂起效缓慢的缺点。OBJECTIVE:To study the inhibitory effects of recombinant adenovirus Ad-GFP-C197,which prepared by adenovirus vector system-loading human telomerase reverse transcriptase(hTERT)C fragment(C197),on the proliferation of 3 kinds of tumor cells in vitro.METHODS:Ad-GFP-C197 was amplified and purified with HEK293 cells.Human gastric cancer cells SGC7901,human breast cancer cells MCF7 and human colorectal cancer cells CaCO2 were infected by Ad-GFP-C197 respectively.Using blank adenovirus carrier(Ad-GFP)as reference,the protein expression of C197 in 3 kinds of tumor cells infected by Ad-GFP-C197 was detected by Western blot assay.The inhibitory effects of Ad-GFP-C197 on 3 kinds of tumor cells were detected by MTT assay.The cell proliferation curve was drawn and the proliferation inhibition rate was calculated.RESULTS:The protein expression of C197 was not detected in 3 kinds of tumor cells infected by Ad-GFP,while significant protein expression of C197 was found in above cells infected by Ad-GFP-C197.The proliferation curves of the 3 kinds of tumor cells infected by Ad-GFP-C197 were significantly inhibited with the time extended,and the proliferation inhibitory rate reached 37.31%-41.42%.CONCLUSIONS:Ad-GFP-C197 shows significant inhibitory effects on the proliferation of SGC7901,MCF7 and CaCO2 cells,which is rapid to make up for the slow effect of other telomerase inhibitors.

关 键 词：人端粒酶逆转录酶 端粒酶抑制剂 重组腺病毒 Ad-GFP-C197 肿瘤细胞 细胞增殖 

分 类 号：R966[医药卫生—药理学] R979.1[医药卫生—药学]