不同激素水平下橡胶树品种云研77-2脱分化培养及污染褐化情况研究  

Dehydration and Pollution Browning Study of Yunyan 77-2 under Different Hormone Levels

作　　者：孙小龙[1] 黄菁[1] 梁国平[1] 宁连云[1] 李玲[1] 田海[1] SUN Xiaolong;HUANG Jing;LIANG Guoping;NING Liangyun;LI Ling;TIAN Hai

机构地区：[1]云南省热带作物科学研究所,云南景洪666100

出　　处：《热带农业科技》2018年第4期16-18,35,共4页Tropical Agricultural Science & Technology

基　　金：云南省热带作物科技创新体系建设专项资金项目(RF2018-1)

摘　　要：为了优化橡胶树脱分化培养体系,以橡胶树品种云研77-2的花药和胚珠作为外植体,在不同激素水平下对其花药和胚珠进行愈伤组织诱导研究,进一步观察其污染与褐化情况。结果表明,2,4-D对花药愈伤组织培养起主导性的作用,橡胶树云研77-2花药愈伤培养最适培养基为改良MS+2.0 mg/L 2,4-D+1.0 mg/L NAA+1.5 mg/L KT。花药、胚珠污染与褐化试验中,胚珠培养污染和褐化率高于花药培养,且细菌、真菌污染率高于褐化率。In order to optimize the dedifferentiation culture system of rubber tree,anther and ovule of Yunyan 77-2 rubber tree species were used as explants to study the induction of anther callus by different hormone levels,and to further observe their pollution browning,The results showed that 2,4-D played a dominant role in anther callus culture.The optimum culture medium of rubber tree Yunyan 77-2 anther culture was MS+2.0 mg/L 2,4-D+1.0 mg/L NAA+1.5 mg/L KT.In anther and ovule browning test,the browning rate of ovule culture was higher than that of anther culture,and the contamination rate of bacteria and fungi was higher than that of browning.

关 键 词：橡胶树 脱分化 污染 褐化 

分 类 号：S794.1[农业科学—林木遗传育种]