等离子体电解氧化涂层钛金属植入物体内骨整合的评价  

Evaluation of osseointegration of plasma electrolytic oxidation coated titanium implants in vivo

作　　者：贺韬[1,2] 张云辉 张超 徐志国[4] 董宇启 He Tao;Zhang Yunhui;Zhang Chao;Xu Zhiguo;Dong Yuqi(Department of Traumatic Orthopedics,Shanghai Renji Hospital,School of Medicine,Shanghai Jiao Tong University,Shanghai 200127,China;Laboratory of Biomechanics and Experimental Orthopaedics,Clinic Gro遠adern,Ludwig-Maximillian University,Munich 81377,Germany;Department of Orthopedics,Shanghai Baoshan District Hospital of Integrated Traditional and Western Medicine,Shanghai 201900,China;Department of Orthopedics,Shandong Energy Zaozhuang Mining Central Hospital,Zaozhuang 277100,Shandong Province,China)

机构地区：[1]上海交通大学医学院附属仁济医院创伤骨科,上海市200127 [2]德国慕尼黑大学附属大哈登医院骨科,生物力学与组织工程实验室,德国慕尼黑81377 [3]上海市宝山区中西医结合医院骨科,上海市201900 [4]山东能源枣矿集团中心医院骨科,山东省枣庄市277100

出　　处：《中国组织工程研究》2018年第34期5469-5476,共8页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(30700170);上海市科委基础重点项目(07JC14057);上海市科委纳米专项项目(0852nm03300);中国国家留学基金管理委员会项目(201606230235)。

摘　　要：背景:多项体外细胞学实验显示,含氧化涂层钛金属较普通钛金属表面表现出更好的生物相容性和生物活性。目的:进一步观察等离子体电解氧化涂层钛棒在动物体内的骨整合能力。方法:取36只16周龄雄性SD大鼠(购自上海西普尔-必凯实验动物有限公司),建立双侧股骨远端髓内钉植入模型,左侧植入多孔纳米等离子体电解氧化涂层钛棒,作为实验组;右侧植入无涂层钛棒,作为对照组。植入2,4,6周时,处死大鼠并收获股骨远端带有金属植入物的骨组织标本,进行Van Gieson染色、双标荧光染色及生物力学评价。结果与结论:(1)Van Gieson染色显示,实验组与对照组植入物-骨组织界面均未见纤维组织长入;植入2周时,两组均可见植入物表面部分骨组织接触,实验组于植入4周时形成植入物周围骨整合,对照组于植入6周时形成植入物周围骨整合;(2)双标荧光染色显示,植入2周时,两组植入物周围均产生大量新生骨组织,与钛棒结合紧密;植入4周时,实验组新生骨与植入物表面呈现连续性接触,对照组新生骨与植入物表面呈不连续区块接触;植入6周时,两组均显示出了连续的植入物-骨组织接触,但新生骨骨量明显较4周时减少;(3)生物力学检测显示,实验组植入4周时的最大压缩载荷高于对照组(P <0.001),两组植入2,6周时的最大压缩载荷比较无差异(P> 0.05);实验组植入4周时的最大压缩载荷高于植入2,6周时(P <0.05),对照组植入6周时的最大压缩载荷高于植入2,4周时(P <0.05);(4)结果表明,等离子体电解氧化涂层钛棒植入早期的骨整合能力优于普通钛棒。BACKGROUND:A number of in vitro cytological experiments have shown that titanium oxide surfaces with oxidized coatings exhibit better biocompatibility and biological activity than conventional titanium metal surfaces.OBJECTIVE:To further observe the osseointegration ability of plasma electrolytic oxidation(PEO)coated titanium rods in vivo.METHODS:Thirty-six 16-week-old male Sprague-Dawley rats(purchased from Shanghai Sippr-BK Laboratory Animal Co.,Ltd.,China)were used to establish a bilateral distal femoral intramedullary nail implantation model.PEO coated titanium rods were implanted into the left side as the PEO group,whilst uncoated titanium rods were implanted into the right side as the control group.At 2,4,and 6 weeks after implantation,the rats were sacrificed and bone specimens with implants at the distal femur were harvested.Van Gieson staining,double-labeled fluorescent staining,and biomechanical evaluation were then performed.RESULTS AND CONCLUSION:(1)Van Gieson staining results showed no fibrous tissue ingrowth at the implant-bone interface in both the experimental group and the control group.At 2 weeks of implantation,bone tissue growth was observed at the implant surface in both groups.The osseointegration around the implant was formed at 4 weeks of implantation in the PEO group,while that of control group was formed at 6 weeks after implantation.(2)Double-labeled fluorescent staining results showed that at 2 weeks after implantation,implants in both groups were closely bound to a large amount of newly formed bone tissue.When implanted for 4 weeks,the newly formed bone in PEO group was in continuous contact with the surface of the implant,while that in the control group was in contact with the surface of the implant in a discontinuous block.At 6 weeks after implantation,continuous implant-bone contact was observed in both groups,but the amount of newly formed bone was significantly lower than that at 4 weeks.(3)Biomechanical examination results showed that the maximum compression load of the PEO grou

关 键 词：等离子体电解氧化涂层 钛金属 植入物 骨整合 双标荧光 生物力学 逆行股骨髓内钉模型 体内实验 生物材料 钛 假体和植入物 骨整合 生物力学 组织工程 

分 类 号：R318[医药卫生—生物医学工程]