采用静电纺丝技术制备丝素纤维蛋白/聚已内酯临时肩袖补片  

Preparation of silk fibroin/polycaprolactone temporary rotator cuff patch by electrospinning technique

作　　者：郭岗岗 庞亚博 杨建华[1] 刘士臣[1] 高爽 肖统光 张伟祥 翟饶生[1] 郭全义[2] Guo Ganggang;Pang Yabo;Yang Jianhua;Liu Shichen;Gao Shuang;Xiao Tongguang;Zhang Weixiang;Zhai Raosheng;Guo Quanyi(Jiamusi University,Jiamusi 154007,Heilongjiang Province,China;Institute of Orthopedics,Chinese PLA General Hospital,Beijing Key Laboratory of Regenerative Medicine,PLA Key Laboratory of Orthopaedic Trauma,Beijing 100853,China;Xuzhou Medical University,Xuzhou 221004,Jiangsu Province,China;Peking University,Beijing 100871,China;Haozhou People’s Hospital,Haozhou 236800,Anhui Province,China;Lanxi People’s Hospital,Lanxi 321100,Zhejiang Province,China)

机构地区：[1]佳木斯大学,黑龙江省佳木斯市154007 [2]解放军总医院骨科研究所,再生医学北京市重点实验室,全军骨科战创伤重点实验室,北京市100853 [3]徐州医科大学,江苏省徐州市221004 [4]北京大学,北京市100871 [5]亳州市人民医院,安徽省亳州市236800 [6]兰溪市人民医院,浙江省兰溪市321100

出　　处：《中国组织工程研究》2018年第34期5501-5509,共9页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金(81472092,81772319);国家重点研发计划课题(2017YFC1104102,2017YFC1103404);北京市自然科学基金(7172203);北京市科技专项(Z161100005016059);佳木斯大学科学技术重点项目(12Z1201507)。

摘　　要：背景:目前肩袖撕裂术后二次断裂率比较高,肩袖补片被设计用于增强损伤肩袖的愈合,因此制备生物相容性好、可降解、生物力学性能好的临时肩袖补片为关键环节。目的:通过静电纺丝技术制备出不同质量比例的丝素纤维蛋白/聚已内酯临时肩袖补片,评估其理化性质和生物相容性。方法:采用静电纺丝技术制备丝素纤维蛋白与聚已内酯质量比分别为4∶0、3∶1、1∶1、1∶3、0∶4的临时肩袖补片,对其微观结构、接触角、降解性能及成分进行表征。将质量比3∶1、1∶1、1∶3、0∶4的临时肩袖补片分别植入SD大鼠背部皮下,植入后2,4周后取补片材料,进行苏木精-伊红染色。结果与结论:(1)混合材料制备的临时肩袖补片,改善了单纯聚已内酯补片的直径、孔径数目、孔径直径及单纯丝素纤维蛋白补片脆性高而致的表面串珠样液滴;(2)混合材料制备的临时肩袖补片呈亲水特性,改善了单纯丝素纤维蛋白补片的高度亲水性及单纯聚已内酯补片的疏水性;(3)在混合材料制备的临时肩袖补片中,两种材料未发生化学反应;(4)除质量比0∶4的临时肩袖补片外,其余质量比临时肩袖补片浸泡于PBS中4周的质量均增加;(5)皮下植入后2周,各组有大量细胞密集黏附在肩袖补片周围,新生组织中有毛细血管形成,肩袖补片外面中间层拥有类似真正肌腱组织的微结构,纯聚已内酯补片外新生组织中层类似正常肌腱组织功能最差;(6)皮下植入后4周,贴附补片新生组织中的细胞数量明显减少,类似正常肌腱组织的外新生组织结构厚度增加,纯聚已内酯补片外新生组织中层类似正常肌腱组织功能最差;(7)结果表明,丝素纤维蛋白改善了聚已内酯补片诱导修复细胞向肌腱细胞分化的潜能。BACKGROUND:As the rate of re-fracture of the rotator is very high,the rotator cuff patch is designed to enhance the healing of the injured rotator cuff.Therefore,the preparation of temporary rotator cuff patch with good biocompatibility,biodegradability and biomechanical properties has become a committed step.OBJECTIVE:To prepare a temporary rotator cuff patch with different ratio of silk fibroin(SF)/polycaprolactone(PCL)using electrospinning technique,and to evaluate its physiochemical properties and biocompatibility.METHODS:Temporary rotator cuff patches with SF/PCL at different mass ratios of 4:0,3:1,1:1,1:3,0:4 were prepared using the electrospinning technique.Microstructure,contact angle,degradation properties and composition of the patches were characterized.Temporary rotator cuff patches with the mass ratios of 3:1,1:1,1:3,and 0:4 were implanted subcutaneously into the back of Sprague-Dawely rats.After 2 and 4 weeks of implantation,the patch material was taken for hematoxylin-eosin staining.RESULTS AND CONCLUSION:(1)The temporary rotator cuff patches prepared from the mixed material improved the diameter,aperture number,and pore diameter of the pure PCL patch as well as the surface droplets caused by the high brittleness of the pure SF patch.(2)The temporary rotator cuff patches prepared by mixed materials improved hydrophilicity as compared with the SF patch and improved hydrophobicity as compared with the simple PCL patch.(3)In the temporary rotator cuff patch prepared by mixed materials,no chemical reaction occurred between the two materials.(4)With the exception of the temporary rotator cuff patch with a mass ratio of 0:4,the rest patches showed an increase in quality after immersed in PBS for 4 weeks.(5)Two weeks after subcutaneous implantation,a large number of cells were attached to the rotator cuff patch in each group,and capillaries formed in the neonatal tissues.The outer middle layer of the rotator cuff patch had a microstructure similar to the normal tendon tissue,while the middle layer of the

关 键 词：肩袖撕裂 丝素纤维蛋白 聚已内酯 静电纺丝 二次撕裂 临时肩袖补片 生物相容性 接触角 红外光谱 扫描电镜 生物材料 纤维蛋白 材料试验 组织工程 

分 类 号：R318[医药卫生—生物医学工程]