电针诱导大麻素受体表达参与展神经修复的相关研究  

Electroacupuncture-induced cannabinoid receptor expression in repair of aortic nerve

作　　者：王蕾 张毅[2] 王旭东[3] 叶子[2] Wang Lei;Zhang Yi;Wang Xudong;Ye Zi(Department of Emergency Center,Second Affiliated Hospital of Nantong University,Nantong 226001,China;Department of Neurosurgery,Second Affiliated Hospital of Nantong University,Nantong 226001,China;Department of Chinese Traditional Medicine,Second Affiliated Hospital of Nantong University,Nantong 226001,China)

机构地区：[1]南通大学第二附属医院急诊中心,南通226001 [2]南通大学第二附属医院神经外科,南通226001 [3]南通大学第二附属医院中医科,南通226001

出　　处：《中华神经创伤外科电子杂志》2018年第5期291-296,共6页Chinese Journal Of Neurotraumatic Surgery：Electronic Edition

基　　金：江苏省科技厅自然科学基金(BK20161290)

摘　　要：目的探讨电针是否通过大麻素受体介导调控小胶质细胞活化促进损伤展神经修复。方法选用健康雄性成年Beagle犬25只随机分为5组:假手术组(A组)、损伤组(B组)、电针处理组(C组)、拮抗剂组(D组)、溶剂组(E组)。将Beagle犬麻醉后,A组仅暴露分离展神经,在其他组电针刺激时进行束缚;B组制作展神经损伤模型,在其他组电针刺激时进行束缚;C组在展神经损伤模型建立后进行电针刺激;D组损伤模型成功后进行AM630注射并予以电针刺激;E组操作同D组,在每次电针刺激前给予溶剂进行腹腔注射。运用Western blot检测A、B、C 3组小胶质细胞大麻素1型受体(CB1R)、CB2R表达,进一步试验取材运用免疫荧光分析5组CB2R表达的情况,并进行统计学差异分析。结果 Western blot检测显示,B、C组分别与A组比较,CB1R蛋白的表达量差异均无统计学意义(P>0.05);电针预处理持续15 min连续2周后,C组CB2R的蛋白表达量显著高于A组,差异有统计学意义(P<0.05),而A组与B组间差异无明显统计学意义(P>0.05)。免疫荧光显示,C、E组分别与A组比较,Arginse/CD11b差异均有统计学意义(P<0.05)。结论 CB2R主要参与电针刺激诱导神经损伤保护作用;电针能够通过CB2R调控小胶质活化状态促进损伤展神经修复。Objective To investigate whether electroacupuncture can promote the repair of injured optic nerve by cannabinoid receptor-mediated regulation of microglia activation.Methods Healthy male Beagle dogs were randomly divided into five groups:sham operation group(A),injury group(B),electroacupuncture treatment group(C),antagonist group(D),and solvent group(E).After Beagle dogs are anesthetized,group A:only the abducens nerve was exposed and separated and bound by electroacupuncture in other groups;group B:Abducens nerve injury model was made and bound by electroacupuncture in other groups;group C:Beagle dogs abducens nerve injury model was established and electroacupuncture stimulation was carried out;group D:AM630 was injected after the injury model was successful and electroacupuncture stimulation was given;group E:They were treated with group D,but before each electroacupuncture stimulation,they were given intraperitoneal injection.Western blot was used to detect the expression of cannabinoid type 1 receptor(CB1R)and CB2R in microglia of group A,B and C.Immunofluorescence was used to analyze the expression of CB2R in 5 groups.Results Compared with group A,Western blot test showed no significant change in the expression of CB1R protein in group C after treatment(P>0.05).At the same time,there was no significant difference in the expression of CB1R protein in the group A compared with the group B(P>0.05).The expression of CB2R protein in group C was significantly higher than that in group A after 15 min continuous electroacupuncture treatment(P<0.01),but there was no significant difference in the expression of CB2R protein in groups A and B.The number of Arginse/CD11b cells in the C group was significantly different from that of the group A(P<0.05),while the number of Arginse/CD11b cells in the group E was also statistically significant compared with that of the group A(P<0.05).Conclusion The CB2R in the cannabinoid receptor is mainly involved in the electroacupuncture-induced neuroprotection.Electroacupunctu

关 键 词：展神经损伤 小胶质细胞 大麻素受体 电针 

分 类 号：R245.31[医药卫生—针灸推拿学]