1,25-二羟维生素D_3对肉鸡生长性能、骨骼矿化及小肠NaPi-Ⅱb和VDR mRNA表达的影响  

Effects of 1,25-dihydroxycholecalciferol on Growth Performance,Bone Mineralization and mRNA Expression of NaPi-Ⅱb and VDR in Small Intestine of Broiler

作　　者：张宁[1,2] 杨雪 张金龙 王志祥[1] 韩进诚 卢红梅[3] 陈村年 ZHANG Ning;YANG Xue;ZHANG Jinlong;WANG Zhixiang;HAN Jincheng;LU Hongmei;CHEN Cunnian(College of Animal Science and Veterinary Medicine,Henan Agricultural University, Zhengzhou 450002,China;;College of Biology and Food Science, Shangqiu Normal University,Shangqiu 476000,China;Henan Zhengbenqingyuan Technology Development Co.,Ltd.,Shangqiu 476000,China)

机构地区：[1]河南农业大学牧医工程学院,郑州450002 [2]商丘师范学院生物与食品学院,商丘476000 [3]河南正本清源科技发展股份有限公司,商丘476000

出　　处：《中国畜牧兽医》2018年第10期2716-2723,共8页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金项目(31101732;U1704107);河南省创新型科技人才队伍建设工程项目(CXTD20130058)

摘　　要：试验旨在研究不同水平1,25-二羟基维生素D3(1,25-(OH)2-D3)对1~14日龄肉鸡生长性能、骨骼矿化及促进十二指肠磷吸收关键基因表达的影响。将180只1日龄罗斯308肉鸡分入3个处理,每处理6个重复,每重复10只。3个处理组分别饲喂3种日粮:在基础日粮中分别添加0、5、10μg/kg 1,25-(OH)2-D3,试验期14d。14日龄屠宰肉鸡,剥离股骨、胫骨及十二指肠1/2处的黏膜;测定肉鸡的生长性能、骨骼矿化相关指标,分析Ⅱb型磷酸钠转运蛋白(NaPi-Ⅱb)、细胞核维生素D受体(nVDR)和细胞膜维生素D受体(mVDR)基因表达情况。结果显示:与基础日粮组相比,添加5μg/kg 1,25-(OH)2-D3组肉鸡体增重、采食量、料重比、股骨和胫骨各指标(重量、长度、直径、灰分重量、灰分含量、钙、磷含量)及十二指肠NaPi-Ⅱb、nVDR和mVDR mRNA表达量均未发生显著变化(P>0.05);添加10μg/kg 1,25-(OH)2-D3显著降低了肉鸡体增重、采食量、股骨和胫骨各指标及十二指肠NaPi-Ⅱb、nVDR和mVDR mRNA表达量(P<0.05),同时料重比显著增加(P<0.05)。综合上述结果,在基础日粮中添加5μg/kg 1,25-(OH)2-D3不会影响1~14日龄肉鸡的生长、骨骼矿化及十二指肠磷吸收关键基因表达;但添加量增至10μg/kg会显著抑制1~14日龄肉鸡生长、骨骼发育,影响十二指肠NaPi-Ⅱb和VDR mRNA表达。The experiment was aimed to study the effects of 1,25-dihydroxycholecalciferol(1,25-(OH)2-D 3)on growth performance,bone mineralization and duodenal phosphate transport gene expression.A total of 180 broilers(1 day old)were divided into 3 treatments with 6 replicates per treatment of 10 chickens per replicate.Three levels of 1,25-(OH)2-D 3 were added to the basic diet:0,5 and 10μg/kg,respectively.Broilers were slaughtered at 14 days of age,and femur,tibia,and duodenal mucosa were collected.Growth performance,bone mineralization and mRNA expressions of sodium phosphate transporter(NaPi-Ⅱb),nuclear vitamin D receptor(nVDR)and membrane vitamin D receptor(mVDR)were analyzed.The results showed that:Compared with the basic group,addition 5μg/kg 1,25-(OH)2-D 3 did not significantly affect the body weight gain,feed intake,feed and gain ration,and the weight,length,diameter,ash weight,ash content,calcium content,and phosphorus content of femur and tibia,and mRNA expression of NaPi-Ⅱb,nVDR and mVDR in the duodenum of broilers from 1 to 14 days of age(P>0.05).Adding 10μg/kg 1,25-(OH)2-D 3 significantly reduced body weight gain,feed intake and feed conversion,the quality of femur and tibia,and the mRNA expression of NaPi-Ⅱb,nVDR,and mVDR in the duodenum of broilers(P<0.05).In conclusion,adding 5μg/kg 1,25-(OH)2-D 3 to normal diets did not affect the growth,bone mineralization or the mRNA expression of NaPi-Ⅱb,nVDR and mVDR in duodenum of broilers.However,the addition of 10μg/kg 1,25-(OH)2-D 3 reduced the growth performance,skeletal mineralization and inhibits duodenal NaPi-Ⅱb,nVDR and mVDR gene expression at the transcriptional level.

关 键 词：1 25-(OH)2-D 3 肉鸡 生长性能 骨骼矿化 NaPi-Ⅱb nVDR MVDR 

分 类 号：S816.71[农业科学—饲料科学]