血管内皮生长因子对兔腹主动脉支架植入后血管内皮功能的影响研究  

Effect of vascular endothelial growth factor on vascular endothelial function after abdominal aortic stent implantation in rabbits

作　　者：苗建波 安少波[1] 徐雷[1] 张晓蕾[1] 胡喜田 吴志红[1] 都伟[1] MIAO Jianbo;AN Shaobo;XU Lei;ZHANG Xiaolei;HU Xitian;WU Zhihong;DU Wei(Department of Cardiovascular Medicine,Shijiazhuang First Hospital, Shijiazhuang,Hebei 050011,China)

机构地区：[1]石家庄市第一医院心血管内科,050011

出　　处：《重庆医学》2018年第29期3739-3742,共4页Chongqing medicine

基　　金：石家庄市直医疗卫生计生单位第二批科学技术研究与发展指导计划课题项目(141462933)

摘　　要：目的探讨血管内皮生长因子(VEGF)对新西兰兔腹主动脉支架植入后血管内皮功能的影响。方法选取健康雄性新西兰白兔35只,建立高脂血症动物模型,处死5只分析组织学形态,将剩余30只随机分为裸金属支架组与裸金属支架+VEGF组,各15只。两组新西兰兔均应用腹主动脉支架植入术治疗,选用裸金属支架,其中裸金属支架+VEGF组加用VEGF。流式细胞检测外周血内皮祖细胞(EPC)水平,电镜下观察血管内皮化程度。比较两组EPC水平与损伤血管再内皮化速率。结果裸金属支架+VEGF组EPC水平明显高于裸金属支架组,差异有统计学意义(P<0.05);且裸金属支架+VEGF组损伤血管再内皮化速率明显高于裸金属支架组。结论 VEGF能够提升EPC水平,促进血管内皮新生,加快损伤血管再内皮化速率。Objective To explore the effect of vascular endothelial growth factor(VEGF)on vascular endothelial function in New Zealand rabbits after abdominal aortic stent implantation.Methods A total of 35 healthy male New Zealand rabbits were selected to establish hyperlipidemia models,and 5 of them were sacrificed to analysing their histological patterns.The other 30 rabbits were randomly divided into the bare metal stent(BMS)group and the BMS+VEGF group,15 cases in each group.Two groups of New Zealand rabbits were treated with abdominal aortic stent.BMS were used,and VEGF was added to the BMS+VEGF group.The level of peripheral blood endothelial progenitor cell(EPC)was detected by using flow cytometry,and the degree of endothelialization was observed under electronic speculum.The level of EPC and the re-endothelialization rate of injured blood vessels were compared between the two groups.Results The level of EPC in the BMS+VEGF group was significantly higher than that in the BMS group,there was statistically significant difference(P<0.05);and the re-endothelialization rate of injured blood vessels in the BMS+VEGF group was higher than that in the BMS group.Conclusion VEGF could increase the level of EPC,promote the regeneration of vascular endothelium,and accelerate the re-endothelialization rate of injured blood vessels.

关 键 词：腹主动脉支架植入术 血管内皮生长因子 内皮 血管 

分 类 号：R453.9[医药卫生—治疗学]