基于叶绿体DNA atp B-rbc L片段的典型樱亚属部分种的亲缘关系及分类地位探讨  

Analysis on Relationship and Taxonomic Status of Some Species in Subg. Cerasus Koehne with Chloroplast DNA atpB-rbcL Fragment

作　　者：朱弘[1] 伊贤贵[1] 朱淑霞 王华辰 段一凡[1] 王贤荣[1] ZHU Hong;YI Xian-Gui;ZHU Shu-Xia;WANG Hua-Chen;DUAN Yi-Fan;WANG Xian-Rong(Co-Innovation Center for Sustainable Forestry in Southern China,College of Biology and the Environment,Nanjing Forestry University,Key Laboratory of State Forestry Administration on Subtropical Forest Biodiversity Conservation,Nanjing 210037)

机构地区：[1]南京林业大学南方现代林业协同创新中心,生物与环境学院,热带森林生物多样性保护国家林业局重点实验室,南京210037

出　　处：《植物研究》2018年第6期820-827,共8页Bulletin of Botanical Research

基　　金：江苏省林业三新工程项目(LYSX[2015]17);江苏省研究生科研创新计划项目(KYCX17_0815);2017年南京林业大学优秀博士创新基金。

摘　　要：蔷薇科典型樱亚属植物(Subg. Cerasus Koehne)(樱花类)具有重要的观赏价值,深入研究其种间亲缘关系及分类地位,对于国产樱属植物系统发育与种质资源创新具有重要的理论指导意义。基于PCR扩增与测序技术研究了从云南、江西、福建采集的典型樱亚属3个种(含变种)的叶绿体DNA非编码区atpB-rbcL片段,结合Gen Bank数据库已经提交的其它4个种的同源序列,共获得7个种的30条序列矩阵,以此构建了单倍型中介网络图与系统发育树,分析和探讨了它们的亲缘关系与分类地位。(1)在atpB-rbcL序列矩阵中,774个有效位点中共发现15个多态性位点,占分析序列的1. 94%,(A+T)含量为70. 50%,存在明显的碱基偏好;(2) DNASP 5共检测到9个单倍型,物种间平均单倍型多样性(H_d=0. 880 5±0. 026),平均核苷酸多态性(π=0. 007 11±0. 000 54),除尾叶樱桃(Prunus dielsiana)外(Hap5～Hap7),其余物种拥有各自独特的1个单倍型,具有较丰富的遗传多样性;(3)综合Network中介图与系统发育重建结果:推测磐安樱桃(P. pananensis)处于较原始的进化阶段,相比尾叶樱桃,与浙闽樱桃(P. schneideriana)亲缘关系较近;长柱尾叶樱桃(P. dielsiana var. longistyla)与尾叶樱桃互为姊妹支,并共同构成独立进化单元。综上,叶绿体atpB-rbcL片段在典型樱亚属下种间的适用效果较好,可以优先选为DNA条形码;分子证据结合形态学特征支持将长柱尾叶樱桃作为尾叶樱桃变种的处理意见,而磐安樱桃的分类地位则需要更多的证据。Subg.Cerasus Koehne of Rosaceae families(Flowering Cherries)has important ornamental value.An in-depth research on the interspecies relationships and their taxonomic status contributes to the development of those cherry species and germplasm resource innovation.in China.The tree species of Subg.Cerasus(including variety)collected from Yunnan,Jiangxi and Fujian were amplified and sequenced,using the atp B-rbc L fragment of noncoding region in chloroplast DNA,and integrated with homologous sequences from four other species committed to database of GenBank.Haplotype of Median-Joining network and the phylogenetic tree were both constructed based on a total of 30 sequence matrix including 7 species,and their relationships and classification status were discussed.(1)There were total 15 polymorphism sites in the 774 valid sites of atp B-rbc L sequence matrix,accounting for 1.94%,and the(A+T)content 70.50%,reflecting an obvious base bias;(2)Total 9 haplotypes were detected by DNASP 5 software,with average Haplotype diversity(H d=0.880 5±0.026)and average nueleotide diversity(π=0.007 11±0.000 54);Except for Prunus dielsiana(Hap5~Hap7),the remaining species had their own unique haplotype,which indicate abundant genetic diversity;(3)After integrating with the results of MJ network as well as reconstruction of the phylogenetic tree,we speculated that P.pananensis was during the original stage of evolution,moreover,it is closely related to P.schneideriana when compare with P.dielsiana;P.dielsiana var.longistyla was a sister branch to P.dielsiana,also together constituted an independent evolutionary unit.With the above results,we believe that the Chloroplast DNA atp B-rbc L fragment is relatively suitable at intergeneric or interspecific levels,which could be selected as DNA bar code in priority.Molecular evidence combined with morphological features support the viewpoint that regarding P.dielsiana var.longistyla as a varity of P.dielsiana,while the taxonomic status of P.pananensis calls for more evidence.

关 键 词：典型樱亚属 CPDNA ATP B-rbc L 单倍型 系统发育 分类地位 

分 类 号：Q949.751.8[生物学—植物学]