山地虎耳草转录组SSR信息分析  

Analysis of SSR Information in Transcriptome Sequences of Saxifraga sinomontana

作　　者：李彦 更吉卓玛 贾留坤 王智华 陈世龙[1] 高庆波[1,3] LI Yan;GENGJI Zhuo-Ma;JIA Liu-Kun;WANG Zhi-Hua;CHEN Shi-Long;GAO Qing-Bo(Key Laboratory of Adaptation and Evolution of Plateau Biota,Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining 810001;University of Chinese Academy of Sciences,Beijing 100039;Key Laboratory of Crop Molecular Breeding of Qinghai Province,Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining 810001)

机构地区：[1]中国科学院高山植物适应与进化重点实验室,中国科学院西北高原生物研究所,西宁810001 [2]中国科学院大学,北京100039 [3]青海省作物分子育种重点实验室,中国科学院西北高原生物研究所,西宁810001

出　　处：《植物研究》2018年第6期939-947,共9页Bulletin of Botanical Research

基　　金：中国科学院“西部之光”人才培养引进计划;中国科学院青年创新促进会项目(2016378)。

摘　　要：利用MISA(MicroSatellite)软件对山地虎耳草转录组拼接序列进行微卫星位点信息分析,为后期SSR标记的开发和物种遗传多样性检测提供候选序列。结果发现,在拼接得到的63 763条Unigene序列中含有4 622个SSR,发生频率为7. 25%,有110种重复基元,平均每10. 00 k B出现一个SSR位点。山地虎耳草转录组序列的SSR主要集中在三核苷酸重复(55. 50%),其次为二核苷酸重复(30. 23%)。二核苷酸重复和三核苷酸重复中的优势重复基元分别为AG/TC和AAG/TTC。二核苷酸重复基元的重复次数类型最多,跨度最大,具有更高的多态性,三核苷酸次之,而四、五、六核苷酸重复类型很少。山地虎耳草转录组SSR以5～9次重复为主,且SSR数量随着重复次数的增加逐渐减少,基序长度主要集中于12～30 bp,多态性均在中等以上。MISA(MicroSatellite)software was used to analyze the microsatellites information from transcriptome sequences of Saxifraga sinomontana J.T.Pan&Gornall,intending to provide candidate sequences for the development of SSR markers and detection of genetic diversity.In total,4 622 SSRs were detected in 63 763 unigenes,which was represented by 110 repeat motifs.The frequency of SSRs was detected to be 7.25%,with an average occurrence of per 10.00 kB in length.The tri-nucleotide was the most abundant repeat motif(55.50%),followed by di-nucleotide(30.23%).The dominant repeat motifs in di-nucleotide and tri-nucleotide were AG/TC and AAG/TTC,respectively.In SSRs,the di-nucleotide possessed the most abundant repeat types,the longest span,as well as higher polymorphism,followed by tri-nucleotide.However,for the tetra-nucleotide,penta-nucleotide and hexa-nucleotide,there were few repeat types.For S.sinomontana,the repeat numbers of SSRs were mainly from five to nine,of which the number gradually decreased along with the repeat numbers increased.Besides,the motif length mostly distributed in 12 to 30 bp,and the polymorphism of SSRs was above medium level.

关 键 词：山地虎耳草 转录组 SSR信息分析 

分 类 号：Q949.751.2[生物学—植物学]