小鼠无创性肺功能的测定及其意义  

The significance of a non-invasive measurement of lung function in mice

作　　者：李树民 张敏 王朋 洪凡 王晨 张悦 耿振洋 杨欣雨 贺笑笑 孙影 杨方 LI Shumin;ZHANG Min;WANG Peng;HONG Fan;WANG Chen;ZHANG Yue;GENG Zhenyang;YANG Xinyu;HE Xiaoxiao;SUN Ying;YANG Fang(School of Public Health,North China University of Science and Technology,Tangshan 063210,China;Research Center of Medical Experiment,North China University of Science and Technology,Tangshan 063210;Physiology Department,School of Basic Medical Sciences,North China University of Science and Technology, Tangshan 063210;Pathology Department,School of Basic Medical Sciences,North China University of Science and Technology,Tangshan 063210,China)

机构地区：[1]华北理工大学公共卫生学院,河北唐山063210 [2]华北理工大学基础医学院生理学系,河北唐山063210 [3]华北理工大学医学实验研究中心,河北唐山063210 [4]华北理工大学基础医学院病理学系,河北唐山063210

出　　处：《中国实验动物学报》2018年第5期548-553,共6页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金(81472953);河北省自然科学基金(H2016209170);华北理工大学大学生创新创业训练计划(X2018351)~~

摘　　要：目的检测C57BL/6小鼠无创性肺功能指标,为小鼠实验性研究肺功能的监测提供依据。方法采用整体体积描记法(whole body plethysmography,WBP)检测460只C57BL/6小鼠肺功能的各项指标,对检测结果进行统计分析,百分位数法确定参考值范围。结果小鼠吸气时间(Ti)64.7(55.30～82.60)ms,呼气时间(Te)83.4(71.70～109.20)ms,呼气峰值时间比率(Rpef)0.21(0.16～0.28),吸气末端暂停(EIP)2.19(1.96～3.76)ms,呼气末暂停(EEP)1.67(0.12～9.15)ms,潮气量(TVb)0.44(0.25～0.58)mL,气道狭窄指数(Penh)1.29(0.91～2.00),呼吸暂停(PAU)1.18(1.00～1.64),呼气中期流速(EF50)0.64(0.30～0.98)mL/s,松弛时间(Tr)39.0(32.40～51.50)ms,最大吸气流速(PIF)9.74(5.33～12.83)mL/s,最大呼气流速(PEF)9.86(5.12～13.47)mL/s,呼吸频率(f)412(331～474)BPM,每分钟通气量(MVb)174.4(86.69～235.04)mL。结论 C57BL/6小鼠无创肺功能指标参考值范围的测定,可为呼吸系统疾病的基础研究提供实验性参考依据。To provide a reference for studies of monitoring lung function in mice,this study was aimed to test the indexes of a non-invasive measurement of lung function in mice.Methods Lung function indexes of 460 C57BL/6 mice were detected by whole-body plethysmography.The results were analyzed,and the range of reference values was determined by the percentile method.Results Normal ranges for the following measures were found:inspiration time was 64.7(55.30-82.60)ms,expiration time was 83.4(71.70-109.20)ms,Rpef was 0.21(0.16-0.28)ms,end-inspiratory pause was 2.19(1.96-3.76)ms,end-expiratory pause was 1.67(0.12-9.15)ms,tidal volume was 0.44(0.25-0.58)mL,enhanced pause was 1.29(0.91-2.00)ms,pause was 1.18(1.00-1.64)ms,expiratory flow-50 was 0.64(0.30-0.98)mL/s,relaxation time was 39.0(32.40-51.50)ms,peak inspiratory flow was 9.74(5.33-12.83)mL/s,peak expiratory flow was 9.86(5.12-13.47)mL/s,inspiratory frequency was 412(331-474)BPM,and minute volume was 174.4(86.69-235.04)mL.Conclusions The normal reference ranges from non-invasive lung monitoring in C57BL/6 mice can be used as a reference for basic research on respiratory diseases.

关 键 词：C57BL/6小鼠 肺功能 整体体积描记法 参考值 

分 类 号：Q95-33[生物学—动物学]