老龄大鼠自发性肝胆管增生的病理学研究  

Pathological examination of spontaneous liver bile duct hyperplasia in aging rats

作　　者：张蕊 易月娥 杜牧 赵蕾 张惠铭 左从林 ZHANG Rui;YI Yuee;DU Mu;ZHAO Lei;ZHANG Huiming;ZUO Conglin(JOINN Laboratories,Beijing 100176,China)

机构地区：[1]北京昭衍新药研究中心,北京100176

出　　处：《中国实验动物学报》2018年第5期561-566,共6页Acta Laboratorium Animalis Scientia Sinica

基　　金："十二五"国家科技重大专项重大新药创制专项项目(2011ZX09301-004)~~

摘　　要：目的为相关科研及新药安全评价工作积累大鼠肝胆管增生有价值的研究资料。方法大鼠共分为3组,第1组雌雄各30只动物(进口SD大鼠);第2组雌雄各60只(国产SD大鼠);第3组雌雄各60只(国产Wistar大鼠)。实验末期,对所有实验动物进行安乐死,进行系统解剖,对肝进行制片,进行组织病理学检查和免疫组织化学研究。结果各组大鼠均发生了不同程度的肝汇管区胆管增生,总发病率是32.33%。其中国产SD大鼠发病率明显高于进口SD大鼠(26.67%∶1.67%);国产Wistar大鼠的发生率明显高于国产SD大鼠(53.33%∶26.67%);雄性动物的发病率明显高于雌性动物(20%∶12.33%)。病理学观察显示多样化的胆管增生和纤维化改变,病变在I级和II级的大鼠发病率是84.5%,III级病变的发病率仅占15.5%。卵圆细胞的增生与胆管增生病变情况相一致,并呈现向胆管上皮方向分化。结论不同种系、不同性别的大鼠间肝胆管增生的发生率存在差异。本研究结果为动物和人类在增龄情况下肝胆管增生的研究提供了参考资料。Objective To examine the incidence and pathological characteristics of spontaneous liver bile duct hyperplasia in aging rats.Methods Rats were divided into three groups:Group 1,30 imported Sprague Dawley(SD)male rats and 30 imported SD females;Group 2,60 domestic SD males and 60 domestic SD females;and Group 3,60 domestic Wistar males and 60 domestic Wistar females.The animals were euthanized after feeding for 104 weeks.The livers were collected for conventional histopathological and immunohistochemical analyses.Results Bile duct hyperplasia was observed in all groups,with an overall incidence rate of 32.33%.However,the incidence rate of bile duct hyperplasia in domestic SD rats was higher than that in the imported SD rats(26.67%vs 1.67%,respectively),the incidence rate was higher in domestic Wistar rats than that in domestic SD rats(53.33%vs 26.67%,respectively),and the incidence rate was higher in the male rats than that in the female rats(20%vs 12.33%,respectively).Multiple morphological bile duct hyperplasia findings with fibrosis were seen by pathological examination.We defined three hyperplasia grades to evaluate the pathological lesions.The incidence rate of lesions in grade I and grade II was 84.5%,while the incidence of lesions in grade III was 15.5%.Hyperplasia of oval cells was observed in cases with bile duct hyperplasia,with differentiation to bile duct epithelium.Conclusions The incidence rate of liver bile duct hyperplasia in aging rats varies by species and sex.These data provide a reference for studies on liver bile duct hyperplasia in ageing animals and humans.

关 键 词：老龄大鼠 自发性胆管增生 病理学 肝 卵圆细胞 SD大鼠 WISTAR大鼠 

分 类 号：Q95-33[生物学—动物学]