棉花GhTGA1与GhTGA9.2基因克隆及表达特征分析  

Cloning and Expression Analysis of GhTGA1 and GhTGA9.2 in Cotton

作　　者：赵曾强 李潇玲[2] 张析[2] 张薇[2] 练文明 ZHAO Zengqiang;LI Xiaoling;ZHANG Xi;ZHANG Wei;LIAN Wenming(Agricultural College,Shihezi University,Shihezi 832000,China;Institute of Biotechnology,Xinjiang Academy of Agricultural Reclamation Sciences,Xinjiang Production &Construction Group Key Laboratory of Crop Germplasm Enhancement and Gene Resources Utilization, Shihezi 832000,China;Agricultural Science Institute,1st Agricultural Production Division,Xinjiang Production and Construction Corps,Aral 843301,China)

机构地区：[1]新疆农垦科学院生物技术研究所,作物种质创新与基因资源利用兵团重点实验室,新疆石河子832000 [2]石河子大学农学院,新疆石河子832000 [3]新疆生产建设兵团第一师农业科学研究所,新疆阿拉尔843301

出　　处：《华北农学报》2018年第5期82-88,共7页Acta Agriculturae Boreali-Sinica

基　　金：国家自然科学基金项目(31260358)

摘　　要：为了揭示棉花GhTGA1与GhTGA9. 2基因的生物学功能,利用RT-PCR技术,从抗枯萎病棉花品种中棉所12号中克隆了TGA转录因子基因GhTGA1与GhTGA9. 2。生物信息学分析表明,2个基因开放阅读框(ORF)长分别为1 281,1461 bp,分别编码405,486个氨基酸。序列分析表明,2个基因均含有b ZIP和DOG1结构域,属于b ZIP亚家族TGA转录因子基因。利用实时荧光定量(qRT-PCR)技术进行表达特征分析表明,GhTGA1基因能被枯萎病菌、乙烯(ET)、茉莉酸(JA)诱导上调表达,水杨酸(SA)诱导后,在各处理时间点,该基因表达量均低于Mock,以上结果表明,GhTGA1基因可能通过参与乙烯(ET)和茉莉酸(JA)信号通路调控棉花对枯萎病的抗性;枯萎病处理后,GhTGA9. 2基因在各时间点表达量均低于Mock,呈下调表达,但能被激素(ET、JA、SA)诱导上调表达。结果表明,GhTGA9. 2基因可能通过激素(ET、JA、SA)途径参与胁迫应答,通过对GhTGA1与GhTGA9. 2基因表达特征分析为后续研究提供基础。GhTGA1 and GhTGA9.2 of transcription factor genes were isolated by RT-PCR technology from Zhongmiansuo 12 cultivar of resistance to Fusarium oxysporum.Sequence analysis showed that the ORF of GhTGA1 and GhTGA9.2 were 1 281 bp and 1 461 bp,respectively.They encoded 405 and 486 amino acids,respectively.GhTGA1 and GhTGA9.2 all contained two conserved bZIP and DOG1 domains.They belonged to the TGA transcription factor gene of bZIP subfamily.The GhTGA1 and GhTGA9.2 expression feature were detected by Real-time quantitative PCR(qRT-PCR),the results showed that GhTGA1 gene was up-regulation expressed with the duration of the treatment of Fov,ethylene(ET)and jasmonic acid(JA).In each treatment time point,the expression of GhTGA1 gene was lower than that of Mock with the treatment of salicylic acid(SA).We speculated that GhTGA1 gene might be involved in ethylene(ET)and jasmonic acid(JA)signaling pathway and regulated resistance to Fusarium oxysporum in cotton;After the treatment of Fusarium oxysporum,the expression of GhTGA9.2 gene was lower than Mock at different treatment time points,and was down-regulated expression.In other hand,it was up-regulation expressed with the duration of the treatment of hormone(ET,JA,SA).It suggested that GhTGA9.2 might participate in stress response through hormone(ET,JA and SA)pathways.The analysis of gene expression characteristics of GhTGA1 and GhTGA9.2 provides a basis for further study.

关 键 词：棉花 TGA转录因子 GhTGA1与GhTGA9.2克隆 表达分析 

分 类 号：Q78[生物学—分子生物学] S562.03[农业科学—作物学]