RM6240型系统在记录诱发场突触后电位中的应用  

Application of RM6240 System in Recording Evoked Field Postsynaptic Potential

作　　者：张顺 赵东芹[1] 王秀松 ZHANG Shun;ZHAO Dongqin;WANG Xiusong(School of Life Science,Shandong Normal University,Jinan 250014,China)

机构地区：[1]山东师范大学生命科学学院,山东济南250014

出　　处：《济南大学学报（自然科学版）》2018年第6期492-496,共5页Journal of University of Jinan(Science and Technology)

基　　金：国家自然科学基金项目(81501149;31501861);山东省自然科学基金项目(ZR2015HQ004;ZR2015CM013)

摘　　要：为了探讨RM6240型生物信号记录系统在记录诱发场突触后电位的应用,以成年雄性Wistar大鼠为模式动物,利用自制的镍铬合金丝电极装置来电刺激其内侧前额叶脑区,记录在伏隔核脑区诱发的场突触后电位;同时,用MATLAB软件和p Clamp10信号分析软件来分析场电位信号数据。结果表明:RM6240系统可记录可靠的内侧前额叶-伏隔核通路诱发场突触后电位,且信号至少能够稳定地维持1 h; MATLAB、p Clamp10软件可提高数据分析的效率。To explore how to record the evoked field postsynaptic potential by using the RM6240 biological signal recording system,adult male Wistar rats were taken as model animals,and custom-made assembly of electrode composed of nickel-chromium wires was used to electrically stimulate the medial prefrontal cortex(mPFC),then the postsynaptic field potential in the nucleus accumbens(NAc)was evoked.MATLAB software and pClamp signal analysis software were used to analyze the field potential data.The results show that the reliable evoked field postsynaptic potentials of mPFC-NAc pathway can be recorded by using the RM6240 system and maintained stably for at least 1 h.The efficiency of data analysis can be improved by MATLAB and pClamp softwares.

关 键 词：生物信号 内侧前额叶 伏隔核 诱发场电位 

分 类 号：Q424[生物学—神经生物学]