骨质疏松症和膝关节骨关节炎共病的蛋白组学分析  

Proteomics analysis of osteoporosis with knee osteoarthritis

作　　者：时刚 张开伟 Shi Gang;Zhang Kaiwei(Bayi Orthopedics Hospital of Chengdu Military Region of PLA,Chengdu 610031,Sichuan Province,China;the First Affiliated Hospital of Guizhou University of Chinese Medicine,Guiyang 550002,Guizhou Province,China)

机构地区：[1]解放军成都军区八一骨科医院,四川省成都市610031 [2]贵州中医学院第一附属医院,贵州省贵阳市550002

出　　处：《中国组织工程研究》2018年第36期5753-5759,共7页Chinese Journal of Tissue Engineering Research

基　　金：贵州省2012年省长基金(071303)~~

摘　　要：背景:鉴于骨质疏松症和骨关节炎以及两者共病的发病率较高,且两者之间的关系目前尚无统一性定论,在综合分析国内外相关研究者对两者的相关性的研究方案后,趋利去弊,课题采取运用蛋白组学技术对骨质疏松症与骨关节炎两者共病的软骨组织进行研究,希望从中找出两者共病的蛋白标志物。为两者共病的相关性研究提供客观的物质基础,为防治骨质疏松症和骨关节炎提供理论依据。目的:利用蛋白组学iTRAQ技术,对骨质疏松症、膝关节骨关节炎和骨质疏松症并膝关节骨关节炎模型的新西兰大白兔膝关节关节软骨组织进行差异蛋白定量分析,从中筛选并鉴定出骨质疏松症和骨关节炎共病的蛋白标志物。方法:制备骨质疏松症、膝关节骨关节炎和骨质疏松症并膝关节骨关节炎动物模型,待造模成功后,提取各组动物膝关节关节软骨样本,运用蛋白组iTRAQ技术对各组样本进行蛋白组定量定性分析,对比各组样本中蛋白质组的差异性,筛选出共病组样本中与各自单病组样本中的蛋白表达量存在明显差异(差异倍数>1.2,P <0.01)的特异性蛋白。再经查阅uniport数据库及相关文献,分析特异性蛋白的特性以及与骨质疏松症和膝关节骨关节炎的相关性,以初步鉴定出骨质疏松症和膝关节骨关节炎共病的蛋白标志物。结果与结论:(1)共筛选出12个骨质疏松症和骨关节炎共病的特异性蛋白:α-2-HS糖蛋白、M型肌酸激酶、纤维蛋白原β链、纤维蛋白原γ链、血清白蛋白、维生素D结合蛋白、N-acylethanolamine酸酰胺酶、促微管聚合蛋白2、Asporin蛋白、髓鞘P0蛋白、Uncharacterizedprotein(G1TQR3)、Uncharacterized protein(U3KLT3);(2)上述12个特异性蛋白在骨质疏松症和骨关节炎共病模型组的蛋白表达量均呈显著性升高;(3)根据12个特异性蛋白各自的生物特性与骨质疏松症和膝骨关节炎的病因病理机制的相互BACKGROUND:Incidence of osteoporosis,osteoarthritis and osteoporotic osteoarthritis is high,and the relationship between osteoporosis and osteoarthritis remains controversial.Therefore,our group designed the study on the cartilages of osteoporotic osteoarthritis by proteomics based on the previous researches,so as to search for the related protein markers.It provides an objective basis for the study on osteoporotic osteoarthritis and provides theoretical basis for the prevention and treatment of osteoporosis and osteoarthritis.OBJECTIVE:To quantitatively analyze the proteins in the knee articular cartilage tissue of New Zealand rabbit models of osteoporosis,knee osteoarthritis and osteoporotic knee osteoarthritis by proteomics iTRAQ technique,and to screen the related protein makers.METHODS:Animal models of osteoporosis,knee osteoarthritis and osteoporotic knee osteoarthritis were established.The knee articular cartilage samples were then collected.Quantitative and qualitative analysis of proteome was conducted on these samples using proteomics iTRAQ technique.The differences of proteome among samples were compared.Specific proteins were selected which had significant differences(difference multiples>1.2,P<0.01)in protein expression level between comorbidity and mono-morbidity groups.In addition,characteristics of proteins as well as their relation with osteoporosis and knee osteoarthritis were analyzed by referring to uniport database and relevant literature in order to preliminarily determine the protein marker of the osteoporosis with knee osteoarthritis.RESULTS AND CONCLUSION:A total of 12 specific proteins were screened:alpha 2-HS glycoprotein,creatine kinase M-type,fibrinogen beta chain,fibrin-ogen gamma chain,serum albumin,vitamin D binding protein,N-acylethanol-amine acid amidase,tubulin polymerization-promoting protein family member 2,asporin protein,myelin protein zero,uncharacterized protein(G1TQR3)and uncharacterized protein(U3KLT3).The above 12 proteins were significantly increased in the osteoporoti

关 键 词：骨质疏松症 骨关节炎 iTRAQ技术 蛋白组学 蛋白标志物 

分 类 号：R318[医药卫生—生物医学工程]