脂肪肝模型大鼠自体原位肝移植后的脂质代谢变化  

Lipid metabolism after autologous orthotopic liver transplantation in rat models of fatty liver

作　　者：丁晨 江艺[2] 潘凡[2] 蔡秋程[2] Ding Chen;Jiang Yi;Pan Fan;Cai Qiucheng(Department of Hepatobiliary Surgery,the Third Affiliated Hospital of Fujian Medical University&Fujian Medical University Union Hospital,Fuzhou 350001,Fujian Province,China;Department of Hepatobiliary Surgery,Fuzhou General Hospital of Nanjing Military Command,Fuzhou 350025,Fujian Province,China)

机构地区：[1]福建医科大学附属第三医院,福建医科大学附属协和医院肝胆外科,福建省福州市350001 [2]解放军南京军区福州总医院肝胆外科,福建省福州市350025

出　　处：《中国组织工程研究》2018年第36期5821-5827,共7页Chinese Journal of Tissue Engineering Research

基　　金：福建省自然科学基金项目(2016J01592),课题名称:脂肪干细胞对大鼠脂肪变性供肝移植术后肝功能的保护作用及其机制研究

摘　　要：背景:研究显示,重度脂肪肝移植后移植物原发性无功能和早期移植物功能不良发生率明显升高,因此何种程度脂肪变性能最大程度提高移植物和受体的存活率,有待进行更深入的研究。目的:探讨不同程度脂肪肝大鼠行自体原位肝移植对脂质代谢的影响。方法:取160只SD大鼠(福建医科大学实验动物中心提供),通过高脂饮食分别建立中、重度脂肪肝模型,每种模型80只大鼠;将中度脂肪肝大鼠随机分2组,模型组(A组)进行自体原位肝移植手术,对照组(C组)不做手术;将重度脂肪肝大鼠随机分2组,模型组(B组)进行自体原位肝移植手术,对照组(D组)不做手术。术前、术后1 d、术后1周、术后3周、术后5周分别进行肝功能、脂质代谢及肝脏组织病理学检查。结果与结论:(1)肝功能:A组谷丙转氨酶、谷草转氨酶水平均于术后1 d达峰值,随后逐渐下降,B组术后5周的谷丙转氨酶、谷草转氨酶水平高于A组;(2)脂质代谢:4组大鼠术后总胆固醇、三酰甘油和低密度脂蛋白水平总体呈下降趋势,高密度脂蛋白、脂联素水平总体呈升高趋势;术后第5周,A组总胆固醇、三酰甘油、低密度脂蛋白水平低于B组(P <0.05),高密度脂蛋白、脂联素水平高于B组(P <0.05);(3)肝脏组织病理学:A组术后1 d肝脏呈中度脂肪变性,术后5周脂肪变性程度逆转;B组术后1 d肝脏呈重度脂肪变性,术后5周呈中、重度脂肪变性,未见明显好转,可见炎症细胞浸润,汇管区内静脉淤血及轻度胆汁淤积;(4)结果表明:中、重度脂肪肝大鼠行自体原位肝移植后,均有不同程度加重脂质代谢紊乱,重度脂肪肝较中度脂肪肝可产生更严重的脂质代谢紊乱且恢复更加缓慢。BACKGROUND:Incidence of primary non-function and early graft dysfunction after severe fatty liver transplantation is obviously increased.Therefore,it remains to be further studied to determine the extent to which the lipogenic properties can maximize the survival rate of grafts and receptors.OBJECTIVE:To investigate the effect of autologous orthotopic liver transplantation on lipid metabolism in fatty liver rats.METHODS:Totally 160 Sprague-Dawley rats were selected to establish moderate and severe fatty liver models through high-fat diet.Eighty rats were selected for each model.The rats with moderate and severe fatty liver were randomly divided into two subgroups,and then received orthotopic liver transplantation(groups A and B)or not(groups B and C).Liver function,lipid metabolism and liver histopathology were examined before surgery,1 day,1,3 and 5 weeks after surgery.RESULTS AND CONCLUSION:Liver function:in the group A,the levels of glutamic-pyruvic transaminase and glutamic oxalacetic transaminase peaked at 1 day postoperatively,and then gradually decreased.The levels of glutamic-pyruvic transaminase and glutamic oxalacetic transaminase in the group B at 5 weeks after surgery were higher than those in the group A(P<0.05).Lipid metabolism:the postoperative total cholesterol,triacylglycerol and low-density lipoprotein levels in each group showed an overall downward trend,and the high-density lipoprotein and adiponectin levels showed an overall upward trend.At 5 weeks after surgery,the total cholesterol,triacylglycerol and low-density lipoprotein levels in the group A were significantly lower than those in the group B(P<0.05),and the high-density lipoprotein and adiponectin levels were higher than those in the group B(P<0.05).Liver histopathology:in the group A,the liver presented moderate steatosis at 1 day after surgery,and the degree of steatosis reversed at 5 weeks after surgery.In the group B,the liver presented severe steatosis at 1 day after surgery,and the liver presented moderate and severe steatosis at

关 键 词：非酒精性脂肪肝 肝移植 自体原位肝移植 缺血再灌注损伤 脂质代谢 脂联素 大鼠 组织构建 

分 类 号：R318[医药卫生—生物医学工程]