针刺脾气虚证模型大鼠“足三里”穴后肠系膜淋巴结免疫功能的变化  

Changes of immune function of mesenteric lymph nodes in rats with spleen deficiency syndrome after acupuncture at Zusanli

作　　者：曾荣华[1] 吴慧婷 周露[1] 欧阳厚淦[1] 高书亮[1] 汪建民[1] 欧阳彦楚 崔田田 Zeng Ronghua;Wu Huiting;Zhou Lu;Ouyang Hougan;Gao Shuliang;Wang Jianmin;Ouyang Yanchu;Cui Tiantian(Jiangxi University of Traditional Chinese Medicine,Nanchang 330004,Jiangxi Province,China)

机构地区：[1]江西中医药大学,江西省南昌市330004

出　　处：《中国组织工程研究》2018年第36期5846-5851,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(81360601);江西省卫计委中医药科研计划项目(2014A075);江西中医药大学校级课题(2013ZR0046)~~

摘　　要：背景:肠道及相关淋巴组织是构成肠道免疫的重要组成部分,在脾虚免疫失衡中肠道免疫起到了重要的调节作用。目的:观察针刺对脾虚症大鼠肠系膜淋巴结组织结构的变化、淋巴细胞增殖活化及巨噬细胞吞噬功能的影响,探讨脾虚与肠道黏膜免疫之间相关性及针刺治疗脾虚证的作用机制。方法:选用36只SPF级雌性SD大鼠(购于安徽医科大学实验动物中心)随机分为3组,模型组及针刺组通过劳倦伤脾加饮食失节多因素复合法建立脾虚大鼠模型,通过脾虚模型体征评分及尿D-木糖排泄率检测证实造模成功后,针刺组大鼠针刺双侧"足三里"穴(1次/d,连续14 d),模型组自然恢复,空白组不予干预。治疗结束后摘取小肠肠系膜淋巴结,采用MTS法测定淋巴细胞增殖分化能力,苏木精-伊红染色法观察肠系膜淋巴结组织形态学改变,Perls stain(普鲁士蓝)染色法检测肠系膜淋巴结巨噬细胞数量。结果与结论:(1)肠系膜淋巴结组织结构改变:空白组结构正常;模型组淋巴结网架结构遭到破坏,淋巴小结等结构不完整,淋巴结副皮质区弥漫性扩大变宽,淋巴结功能亢进;针刺组淋巴结小梁构成的实质网架结构基本趋于完整,淋巴小结等结构基本恢复;(2)肠系膜淋巴结淋巴细胞增殖功能:T淋巴细胞及B淋巴细胞的增殖转化呈相同的趋势,但T淋巴细胞增殖率要高于B淋巴细胞(P <0.01);模型组的T、B淋巴细胞增殖率均低于空白组(P <0.01);针刺组T淋巴细胞增殖率高于模型组(P <0.01),与空白组比较差异无显著性意义(P> 0.05);针刺组B淋巴细胞增殖率低于空白组(P <0.01),与模型组比较差异无显著性意义(P> 0.05);(3)巨噬细胞计数:针刺组的淋巴窦巨噬细胞分部密度高于模型组,低于空白组(P <0.01)。提示:针刺"足三里"穴能激活脾虚证大鼠肠系膜淋巴结免疫应答,增强其淋巴细胞的增殖分化及巨噬细胞的吞噬活化能力(其中对BACKGROUND:Intestinal tract and lymphoid tissue are important parts of intestinal immunity,and intestinal immunity plays a critical role in spleen deficiency.OBJECTIVE:To observe the change of the structure of mesenteric lymph node tissues,lymphocyte proliferation and activation,and macrophage phagocytosis in rats with spleen deficiency syndrome,and to explore the correlation between spleen deficiency and intestinal mucosal immunity and the mechanism of acupuncture for spleen deficiency syndrome.METHODS:Thirty-six female Sprague-Dawely rats of SPF grade provided by the Experimental Animal Center,Anhui Medical University were randomly divided into three groups:model group,acupuncture group and blank control group.A rat model of spleen deficiency was established by compounding fatigue and spleen-inflicted and eating disorders.The urine D-xylose excretion rate was measured during the model establishment and during the treatment period.After the model was successfully established,rats in the acupuncture control group were acupunctured at bilateral Zusanli(St36),once daily for 14 consecutive days.The model group naturally recovered,and the blank control group received no intervention.At the end of treatment,mesenteric lymph nodes were harvested and the ability of proliferation and differentiation of lymphocytes was measured by MTS method.Morphological changes of mesenteric lymph nodes were observed by hematoxylin-eosin staining.Perls Prussian blue stain staining was used to detect the number of mesenteric lymph node macrophages.RESULTS AND CONCLUSION:Mesenteric lymph node tissue changes showed that:in the blank control group,the structure was complete.In the model group,the mesenteric lymph node network structure destroyed,lymph nodes and other structures were incomplete,lymph node subcortical area was in diffuse enlargement and widened,showing hyperfunction.In the acupuncture group,the mesenteric lymph node trabecula showed a substantial network structure,and the structure of lymphoid nodules basically recovered.The

关 键 词：脾虚 针刺 肠系膜淋巴结 肠道免疫 组织构建 

分 类 号：R318[医药卫生—生物医学工程]