中等强度有氧运动和右丙亚胺治疗减少表柔比星致大鼠心肌损伤的影响研究  

Effects of moderate-intensity aerobic exercise and dexrazoxane on myocardial injury induced by epirubicin in rats

作　　者：边林莉[1] 方红明[1] 陈峰[1] BIAN Lin-li;FANG Hong-ming;CHEN Feng(Cancer Center of Zhejiang Xiaoshan Hospital,Hangzhou,Zhejiang 311200,China)

机构地区：[1]浙江萧山医院肿瘤中心,浙江杭州311200

出　　处：《预防医学》2018年第11期1095-1099,共5页CHINA PREVENTIVE MEDICINE JOURNAL

基　　金：杭州市科技局资助项目(20163501Y83)

摘　　要：目的探讨中等强度有氧运动(MIAE)联合右丙亚胺(DEX)治疗减少表柔比星致大鼠心肌损伤的作用。方法选取SD雌性大鼠40只,随机分为正常对照组8只和心肌损伤组32只;建立表柔比星致大鼠心肌损伤模型;造模成功后,心肌损伤组随机分为模型组、 DEX组、 MIAE组和DEX+MIAE组;治疗6周后,检测各组大鼠血清过氧化氢酶(CAT)、总超氧化物歧化酶(T-SOD)和丙二醛(MDA)水平,并取大鼠心室组织做病理学检查,评估心肌细胞凋亡情况。结果治疗6周后, DEX+MIAE组CAT水平低于正常对照组,但高于DEX组、 MIAE组和模型组(P<0.05);DEX+MIAE组T-SOD水平低于正常对照组,但高于DEX组、 MIAE组和模型组(P<0.05); DEX+MIAE组MDA水平低于DEX组、 MIAE组和模型组(P<0.05),与正常对照组比较,差异无统计学意义(P>0.05); DEX+MIAE组心肌细胞病理学评分和凋亡指数均低于模型组(P<0.01),但与正常对照组比较,差异无统计学意义(P>0.01)。光镜下观察DEX+MIAE组大鼠心肌细胞病变程度最轻。结论中等强度有氧运动可通过提高CAT和T-SOD活性,降低MDA水平,减少氧自由基产生,实现保护心肌细胞的作用,且提高右丙亚胺治疗效果。Objective To explore the effects of moderate-intensity aerobic exercise(MIAE)and dexrazoxane(DEX)on rats myocardial damage induced by epirubicin.Methods A total of 40 SD female rats were randomly divided into normal control group(n=8)and myocardial injury group(n=32)to establish the rats myocardial damage model-induced by epirubicin.After successful modeling,the myocardial injury group was randomly divided into four groups:model group,DEX group,moderate-intensity aerobic exercise(MIAE)group and dexrazoxane plus moderate-intensity aerobic exercise(DEX+MIAE)group,and each group was treated for six weeks.Catalase(CAT),total superoxide dismutase(T-SOD),malondialdehyde(MDA)and apoptotic myocardial cell index were measured and the ventricular tissue was also pathological examined.Results After six weeks intervention,the level of CAT in the DEX+MIAE group was lower than that in the normal control group,but was higher than that in the DEX group,the MIAE group and the model group(P<0.05);the DEX+MIAE group was lower than the normal control group,but was higher than the DEX group,the MIAE group and the model group in the level of the T-SOD(P<0.05);the DEX+MIAE group was lower than the DEX group,the MIAE group and the model group(P<0.05),but there was no significant difference compared with the normal control group in the level of the MDA(P>0.05).The myocardial cell pathology score and the apoptotic index in the DEX+MIAE group was lower than those in the model group(P<0.01),but there was no significant difference compared with the normal control group(P>0.01).The degree of myocardial cell damage in the DEX+MIAE group was the lightest when observed by light microscope.Conclusion MIAE can improve the activity of CAT and T-SOD,reduce the level of MDA and the production of oxygen free radicals,also protect the cardiomyocytes.MIAE can improve the therapeutic effect of DEX.

关 键 词：中等强度有氧运动 右丙亚胺 表柔比星 心肌损伤 

分 类 号：R542.2[医药卫生—心血管疾病]