转桃PpCuZnSOD基因大豆的耐旱性  

Drought tolerance of transgenic soybean with PpCuZnSOD gene

作　　者：杨艳丽 杨勇[2] 李大红 李鸿雁 YANG Yan-li;YANG Yong;LI Da-hong;LI Hong-yan(School of Biotechnology and Food Engineering,Huanghuai University,Zhumadian 463000,China;Garden Center,Huanghuai University,Zhumadian 463000,China)

机构地区：[1]黄淮学院生物与食品工程学院,河南驻马店463000 [2]黄淮学院园林中心,河南驻马店463000

出　　处：《江苏农业学报》2018年第5期978-983,共6页Jiangsu Journal of Agricultural Sciences

基　　金：河南省科技计划项目(182102110305);河南省高等学校重点项目(16A210032)

摘　　要：为探索桃(Prunus persica L.)铜/锌超氧化物歧化酶基因(PpCuZnSOD)在植物抗干旱胁迫中的作用,应用农杆菌介导法,将PpCuZnSOD基因转入大豆品种中黄13中。Southern印迹分析证实PpCuZnSOD基因已整合到大豆基因组中。定量PCR分析结果表明,转基因大豆中PpCuZnSOD表达水平均明显增加。用15%PEG4000模拟干旱胁迫时,转基因大豆种子萌发率与主根长显著高于非转基因大豆。在干旱10 d条件下,转基因大豆与非转基因大豆相比,超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)活性增加,而丙二醛(MDA)含量下降,叶绿素含量降低较少。活性氧(ROS)染色结果显示,转基因植株在干旱胁迫下活性氧少于非转基因植株。复水后4 d,转基因大豆的成活率显著高于非转基因大豆。这些结果表明,PpCuZnSOD能提高大豆的耐旱性。To explore the role of copper/zinc superoxide dismutase gene(PpCuZnSOD)in drought stress in plants,PpCuZnSOD gene from peach(Prunus persica L.)was transferred into soybean(Gycine max(L.)Merr.)by agrobacterium-mediated transformation.Integration of PpCuZnSOD gene was confirmed by southern blot analysis.The expression levels of PpCuZnSOD in transgenic plants were significantly increased via real time PCR.When the soybean seeds were treated with 15%PEG4000,the seed germination rate and main root length of transgenic soybean were significantly higher than those of non-transgenic soybean(wild type,WT)plants.Under the condition of drought stress for 10 d,the activities of SOD(superoxide dismutase),CAT(catalase)and POD(peroxidase)increased,while malondialdehyde(MDA)content decreased and chlorophyll content decreased slightly in transgenic soybean compared with those in WT.The results of reactive oxygen species(ROS)staining showed that transgenic plants had less reactive oxygen species under drought stress than those of WT.After four days of rehydration,the survival rate of transgenic soybean was significantly higher than that of WT.In conclusion,PpCuZnSOD plays on important role in enhancing the drought tolerance of soybean.

关 键 词：转基因 耐旱性 大豆 PpCuZnSOD基因 

分 类 号：S565.103.53[农业科学—作物学]