百合转录辅激活因子LlMBF1c基因的克隆与表达分析  

Cloning and expression analysis of LlMBF1c in lily

作　　者：曹兴[1] 郭尚敬[1] 高祥斌[1] 吕福堂[1] 隋娟娟[2] 穆红梅[1] 吴泽 义鸣放 张秀省[1] CAO Xing;GUO Shang-jing;GAO Xiang-bin;LYU Fu-tang;SUI Juan-juan;MU Hong-mei;WU Ze;YI Ming-fang;ZHANG Xiu-sheng(College of Agriculture,Liaocheng University,Liaocheng 252059,China;College of Biology and Food Engineering,Fuyang Normal College,Fuyang 236037,China;Beijing Key Laboratory of Development and Quality Control of Ornamental Crops,College of Horticulture,China Agricultural University,Beijing 100193,China)

机构地区：[1]聊城大学农学院,山东聊城252059 [2]阜阳师范学院生物与食品工程学院,安徽阜阳236037 [3]中国农业大学园艺学院,花卉发育与品质调控北京市重点实验室,北京100193

出　　处：《江苏农业学报》2018年第5期1120-1127,共8页Jiangsu Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(31601788);山东省自然科学基金项目(ZR2016CB36);聊城大学博士科研启动基金项目(318051312)

摘　　要：为了深入研究百合响应高温胁迫的分子机制,本研究以麝香百合杂种系品种白天堂为试验材料,从中分离得到1个MBF1c基因,其开放阅读框为438 bp,编码145个氨基酸,蛋白质分子量为15 900,理论等电点为10. 22。多序列同源比对和进化树分析表明百合MBF1c具有典型的MBF1和HTH结构域,归属c亚型MBF1,因此将克隆到的基因命名为LlMBF1c。亚细胞定位结果表明,LlMBF1c可能介导了热激信号由细胞质向细胞核的转导。实时荧光定量PCR分析结果表明,LlMBF1c在叶中的表达量最高,根中次之,鳞茎中最低; LlMBF1c的表达受热激诱导。表明,LlMBF1c基因可能与百合的热激反应密切相关。To explore the molecular mechanism of lily in response to heat stress,the MBF1c homologous gene was isolated from lily cultivar White Heaven.The open reading frame of LlMBF1c gene was 438 bp,a protein of 145 amino acid residues was encoded,and the molecular weight of LlMBF1c protein was 15 900 with a theoretical isoelectric point of 10.22.Sequence alignment and phylogenetic relationship analysis results showed that LlMBF1c belonged to c subtype MBF1,containing a typical MBF1 domain and a HTH domain.Subcellular localization assay results indicated that LlMBF1c could mediate the transduction of heat stress signal from cytoplasm to nucleus.The qRT-PCR results showed that the relatively highest expression level of LlMBF1c was detected in leaf and LlMBF1c transcripts were induced by heat shock.In conclusion,LlMBF1c might be closely related to heat stress response in lily.

关 键 词：百合 MBF1 基因克隆 亚细胞定位 表达分析 

分 类 号：Q785[生物学—分子生物学]