基于SLAF-seq技术的烟草抗CMV主效QTL定位  

CMV Resistance QTL Analysis in Tobacco Based on SALF-seq Technique

作　　者：潘旭浩[1] 程立锐[1] 陈小翠 蒋彩虹[1] 任民[1] 杨爱国[1] PAN Xuhao;CHENG Lirui;CHEN Xiaocui;JIANG Caihong;REN Ming;YANG Aiguo(Tobacco Research Institute,Chinese Academy of Agricultural Sciences,Qingdao 266101,China;Zunyi Agriculture Science Research Institute,Zunyi,Guizhou 563006,China)

机构地区：[1]中国农业科学院烟草研究所,烟草行业烟草基因资源利用重点实验室,青岛266101 [2]贵州省遵义市农业科学研究院,遵义市作物基因资源与种质创制重点实验室,贵州遵义563006

出　　处：《中国烟草科学》2018年第5期1-8,共8页Chinese Tobacco Science

基　　金：中国烟草总公司重大专项项目“烟草抗CMV和赤星病连锁分子标记开发及NC82、K326品种抗性改良”(110201301009,JY-09);“以K326为主要底盘品种的烟草全基因组模块构建”(110201601028,JY-02);中国烟草总公司科技重点项目“烤烟抗主要病毒病(TMV、CMV、PVY)基础材料创制及育种利用”(110201402006)。

摘　　要：烟草花叶病(CMV)是烟草主要病害之一,筛选与CMV抗病QTL紧密连锁的分子标记是烟草抗病分子育种的重要部分。本研究以抗病材料台烟8号和感病材料NC82为亲本,获得BC1群体,构建抗、感池。采用BSA和SLAF-seq技术开发CMV抗病相关SNP分子标记,共获得180 370个SLAF标签,经分析后获得6156个多态性SNP标记。利用SNP-index分析方法,发现两个与CMV抗病高度关联的区域。利用这些分子标记成功定位到了两个CMV抗病相关QTL,其中一个QTL定位于Chr01上的55.72～60.44 Mb区间内,区间大小为4.72 Mb;另一个则定位于烟草Chr18上的44.21～48.70 Mb区间内,区间大小为4.49 Mb。在关联到的QTL区间内设计分子标记可以用于烟草抗CMV育种的辅助选择。Cucumber mosaic virus(CMV)is one of most important diseases in tobacco,and selection of molecular markers closely linked to CMV resistant QTLs is a key for tobacco CMV-resistance breeding.In order to identify genes closely linked to CMV resistance and develop resistant varieties,a BC1 population derived from resistant-parent Taiyan-8 and susceptible-parent NC82 was used to construct resistant and susceptible pools.By application of BSA and SLAF-seq technique,a total of 180,370 SALF labels were obtained and 6,156 polymorphic SNP markers were developed.As a result,two CMV-associated regions were identified by analysis of SNP-index data.Our results showed that abundant polymorphic SNPs can be developed between tobacco varieties using SALF-seq.By utilization of these SNPs,two CMV-resistance QTLs were successfully identified in Chr01 and Chr18,with 4.72 Mb and 4.49 Mb interval,respectively.The two identified QTLs are useful for in molecular marker assisted selection in tobacco breeding programs for CMV resistance.

关 键 词：烟草 CMV抗性 QTL SALF-seq 

分 类 号：S572.03[农业科学—烟草工业]