不同浓度的完全弗氏佐剂影响大鼠疼痛行为的观察  

Observation of the Effect of Different Concentrations of Complete Freund's Adjuvant on Pain Behavior in Rats

作　　者：贾欣[1] 代洁琼 乔巧玉 赵欣[1] 王志华[1] 关晓雅 杜振莹 张宇[1] JIA Xin;DAI Jieqiong;QIAO Qiaoyu(Shanxi Medical University,Taiyuan 030001,China)

机构地区：[1]山西医科大学,山西太原030001

出　　处：《中国医学创新》2018年第29期1-5,共5页Medical Innovation of China

基　　金：国家自然科学基金(81371254)

摘　　要：目的:观察脚掌注射不同浓度完全弗氏佐剂(complete Freund’s adjuvant,CFA)产生的不同强度的伤害刺激对大鼠痛感受和痛情绪反应程度的影响。方法:福尔马林诱导的条件性位置回避(conditioned place avoidance,CPA)的行为测试近来被用于评估痛情绪反应。本实验采用另一种炎性致痛物质CFA在大鼠的脚掌皮下注射使之产生持续性炎性痛,诱导大鼠产生条件性位置回避(conditioned place avoidance,C-CPA)反应。根据在大鼠的脚掌皮下注射不同浓度的CFA将大鼠分为正常(Naive)组,生理盐水(NS)组,1/5 CFA组,1/3 CFA组,1/2 CFA组,之后分别检测各组大鼠的热缩足潜伏期(paw withdrawal latency,PWL)和C-CPA反应。结果:1/3 CFA组和1/2 CFA组匹配后热缩足潜伏期与匹配前比较,均明显减小,即痛阈下降,且两组匹配前与匹配后的差值与生理盐水组相比,差异均有统计学意义(P<0.05);1/3 CFA组的下降值小于1/2 CFA组(P<0.05);1/2 CFA组在匹配后痛侧停留的时间明显缩短,且回避分数与生理盐水组比较,差异有统计学意义(P<0.05)。结论:CFA所诱导的PWL反应和CPA反应的程度与CFA的注射浓度有关,从行为上证实大鼠痛情绪与痛感受的产生存在不同的机制。Objective:To observe the effect of injurious stimulation of different intensity produced by injecting different concentrations of CFA into the left hind paw on pain emotion and pain perception response in rats.Method:Behavioral testing of conditioned place avoidance(CPA)induced by formalin has recently been used to assess pain emotion response.In this study,another inflammatory pain substance CFA was injected in the left hind paw of rats to produce persistent inflammatory pain,and the rats were induced to produce conditioned place avoidance reaction(C-CPA).Rats were divided into normal(Naive)group,normal saline(NS)group,1/5 CFA group,1/3 CFA group,and 1/2 CFA group according to subcutaneous injection of different concentrations of CFA in the rat’s paw.Then the thermal withdrawal paw latency(PWL)and C-CPA response of every group rats were tested.Result:The incubation period of thermoplastic foot of 1/3 CFA group and 1/2 CFA group after matching were significantly reduced,that is,the pain threshold was decreased,and the difference between the two groups before and after matching was statistically significant compared with the saline group(P<0.05);the 1/3 CFA group decreased less than that of the 1/2 CFA group(P<0.05).The duration of pain side stay in the 1/2 CFA group was significantly shortened after matching,and the avoidance score was statistically significant compared with that in the saline group(P<0.05).Conclusion:The extent of PWL response and CPA response induced by CFA was related to the injection concentration of CFA,and it was confirmed from the behavior that different mechanisms exist for the generation of pain emotion and pain perception.

关 键 词：完全弗氏佐剂 条件性位置回避 前扣带皮层 痛情绪 

分 类 号：R402[医药卫生—临床医学] R-332