Effect of a Single Repeat Sequence of the Human Telomere d（TTAGGG）on Structure of Single-Stranded Telomeric DNA d[AGGG（TTAGGG）6]  

单个人类端粒重复序列d（TTAGGG）对长链端粒DNA-d[AGGG（TTAGGG）6]结构影响的研究（英文）

作　　者：Tian-lei Guang Ya-ting Gao Xiao-dong Ye 光天磊;高亚婷;叶晓东(中国科学技术大学化学物理系,合肥微尺度物质科学国家研究中心,合肥230026;中国科学技术大学,中国科学院软物质化学重点实验室,合肥230026)

机构地区：[1]Hefei National Laboratory for Physical Sciences at the Microscale,Department of Chemical Physics,University of Science and Technology of China,Hefei 230026,China [2]CAS Key Laboratory of Soft Matter Chemistry,University of Science and Technology of China,Hefei 230026,China

出　　处：《Chinese Journal of Chemical Physics》2018年第5期635-640,735,共7页化学物理学报（英文）

基　　金：supported by the National Natural Scientific Foundation of China (No.21674107);the Fundamental Research Funds for the Central Universities (No.WK2340000066)

摘　　要：The structures of human telomeric DNA have received much attention due to its significant biological importance.Most studies have focused on G-quadruplex structure formed by short telomeric DNA sequence,but little is known about the structures of long singlestranded telomeric DNAs.Here,we investigated the structure of DNA with a long sequence of d[AGGG(TTAGGG)6](G6-DNA)and the effect of a single repeat sequence d(TTAGGG)(G01-DNA)on the structure of G6-DNA using sedimentation velocity technique,polyacrylamide gel electrophoresis,circular dichroism spectroscopy,and UV melting experiments.The results suggest that the G6-DNA can form dimers in aqueous solutions and G01-DNA can form additional G-quadruplex structures by binding to G6-DNA.However,G01-DNA has no effect on the structure of DNA with a sequence of d[AGGG(TTAGGG)3](G3-DNA).Our study provides new insights into the structure polymorphism of long human single-stranded telomeric DNA.

关 键 词：Circular dichroism spectroscopy G-guadruplex Sedimentation velocity experiments 

分 类 号：Q75[生物学—分子生物学]