蒙药肉豆蔻-8散对实验动物心脏的保护作用研究  

Study on the Protective Effects of Mongolian Medicine Roudoukou-8 Powder on Cardiac Tissue of Experimental Animals

作　　者：肖云峰[1] 李文妍 王玉华[3,4] XIAO Yunfeng;LI Wenyan;WANG Yuhua(Center for New Drug Safety Evaluation and Research,Inner Mongolia Medical University,Hohhot 010110,China;Dept.of Pharmacy,Inner Mongolia People’s Hospital,Hohhot 010017,China;TCM Research Institute,Tianjin University of TCM,Tianjin 300193,China;School of Pharmacy,Inner Mongolia Medical University,Hohhot 010110,China)

机构地区：[1]内蒙古医科大学新药安全评价研究中心,呼和浩特010110 [2]内蒙古自治区人民医院药学处,呼和浩特010017 [3]天津中医药大学中药研究院,天津300193 [4]内蒙古医科大学药学院,呼和浩特010110

出　　处：《中国药房》2018年第21期2944-2948,共5页China Pharmacy

基　　金：国家自然科学基金资助项目(No.81360678);内蒙古自治区高等学校"青年科技英才支持计划"项目(No.NJYT-17-B29);内蒙古医科大学科技百万工程项目(No.YKD2016KJBW013);内蒙古医科大学博士启动基金项目(No.YKD2017BQ001);内蒙古自治区卫生和计划生育委员会医疗卫生科研计划项目(No.201702059)

摘　　要：目的:研究蒙药肉豆蔻-8散对实验动物心脏的保护作用,为其临床应用提供实验依据。方法:以复方丹参滴丸为阳性对照(0.10 g/kg),采用呼吸耗氧法考察低、中、高剂量肉豆蔻-8散(0.75、1.50、3.00 g/kg)连续灌胃给药10 d(每天给药1次)对正常小鼠和盐酸异丙肾上腺素致急性心肌缺血缺氧模型小鼠存活时间的影响。以复方丹参滴丸为阳性对照(0.04 g/kg),考察低、中、高剂量肉豆蔻-8散(0.28、0.56、1.12 g/kg)连续灌胃给药10 d(每天给药1次)对氯仿-肾上腺素致急性心律失常模型兔心律失常潜伏期、心律失常持续期的影响,以及对垂体后叶素致急性心肌损伤模型兔心脏的T波波幅、S-T段波幅和血清中内源性物质[乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)、丙二醛(MDA)和肌酸激酶(CK)]水平的影响。各实验均设置相应的空白对照和模型对照(均给予0.5%羧甲基纤维素钠)。结果:复方丹参滴丸和中、高剂量肉豆蔻-8散均能显著延长正常小鼠和急性心肌缺血缺氧模型小鼠的存活时间(P<0.05或P<0.01)。复方丹参滴丸和高剂量肉豆蔻-8散均能显著延长兔的心律失常潜伏时间(P<0.05),显著缩短兔的心律失常持续时间(P<0.05)。复方丹参滴丸和中剂量肉豆蔻-8散均能显著降低急性心肌损伤模型兔心脏的ΣT波增幅(P<0.05或P<0.01),复方丹参滴丸和中、高剂量肉豆蔻-8散均能显著降低急性心肌损伤模型兔心脏的ΣS-T段波幅(P<0.05或P<0.01);复方丹参滴丸和低、高剂量肉豆蔻-8散均能显著降低急性心肌损伤模型兔血清中LDH水平(P<0.05),中、高剂量肉豆蔻-8散分别能显著降低急性心肌损伤模型兔血清中MDA、CK水平(P<0.05),中、高剂量肉豆蔻-8散均能显著升高急性心肌损伤模型兔血清中SOD水平(P<0.05)。结论:蒙药肉豆蔻-8散对实验动物的心脏有保护作用。OBJECTIVE:To study the protective effect of Mongolian medicine Roudoukou-8 powder on cardiac tissue of experimental animals.METHODS:Using Compound danshen dripping pill as positive control(0.10 g/kg),the experimental animals were given low-dose,medium-dose and high-dose of Roudoukou-8 powder(0.75,1.50,3.00 g/kg)intragastrically for 10 d once a day,and respiratory oxygen consumption method was used to investigate its effects on survival time of normal mice and isoproterenol hydrochloride-induced acute myocardial ischemia and hypoxia model mice.Using Compound danshen dripping pills as positive control(0.04 g/kg),the experimental animals were given low-dose,medium-dose and high-dose of Roudoukou-8 powder(0.28,0.56,1.12 g/kg)intragastrically for 10 d once a day,and its effects on arrhythmia latency and arrhythmia duration of chloroform-epinephrine induced arrhythmia model rabbits was investigated as well as its effects on T wave amplitude,S-T amplitude and serum endogenous substance(LDH,SOD,MDA,CK)levels of cardiac tissue in pituitrin-induced acute myocardial injury model rabbits.The corresponding blank control and model control were provided in all experiments.RESULTS:Compound danshen dripping pill,medium-dose and high-dose of Roudoukou-8 powder could significantly prolong survival time of normal mice,acute myocardial ischemia and hypoxia model mice(P<0.05 or P<0.01).Compound danshen dripping pill and high-dose of Roudoukou-8 powder could significantly prolong arrhythmia latency of rabbits(P<0.05),and also significantly shortened arrhythmia duration(P<0.05).Compound danshen dripping pill and medium-dose of Roudoukou-8 powder could significantly decrease the increase ofΣT wave amplitude in cardiac tissue of acute myocardial injury model rabbits(P<0.05 or P<0.01).Compound danshen dripping pill,medium-dose and high-dose of Roudoukou-8 powder could significantly decrease the increase ofΣT wave amplitudein cardiac tissue of acute myocardial ischemia model rabbits(P<0.05 or P<0.01).Compound danshen dripping pill,low-dos

关 键 词：蒙药 肉豆蔻-8散 耐缺氧 急性心律失常 急性心肌缺血缺氧 小鼠 兔 

分 类 号：R285.5[医药卫生—中药学]