Corin基因通过NF-κB信号通路对心肌细胞增殖凋亡的机制研究  

Influence of Corin gene on proliferation and apoptosis of cardiomyocytes through nuclear factor-κB signaling pathway: a study on mechanism

作　　者：李岩松 姜衡[1] 费民忠[1] 夏静雯[2] 魏永[2] Li Yansong;Jiang Heng;Fei Minzhong;Xia Jingwen;Wei Yong(Department of Cardiovascular Medicine,Central Hospital of Songjiang District,Shanghai 201600,China)

机构地区：[1]上海市松江区中心医院心血管内科,上海201600 [2]上海中医药大学附属第七人民医院心血管内科,上海200137

出　　处：《中国循证心血管医学杂志》2018年第10期1228-1231,1235,共5页Chinese Journal of Evidence-Based Cardiovascular Medicine

基　　金：上海市卫生和计划生育委员会项目资助(201640244)

摘　　要：目的探讨Corin基因通过核转录因子-κB(NF-κB)信号通路对心肌细胞增殖凋亡的影响。方法通过脂质体LipofectamineTM2000将过表达Corin转染H9c2细胞及H2O2刺激细胞,细胞分为Control组、pcDNA3.1组、H2O2组(150μmol/L的H2O2处理细胞6 h)和pcDNA3.1-Corin组(转染pcDNA3.1-Corin后再用H2O2处理细胞6 h),BAY 11-7082作为NF-κB信号通路抑制剂,各组细胞活力通过CCK8法检测,凋亡率通过流式细胞仪检测,通过Western blotting检测各组细胞中增殖相关蛋白ki67、凋亡相关蛋白p53及NF-κB信号通路NF-κB和IκBα的蛋白表达。结果 H2O2刺激的H9c2细胞中Corin的表达明显降低,过表达Corin后Corin的表达显著升高;与pcDNA3.1组比较,H2O2组细胞活力及ki67和IκBα的表达均显著降低,凋亡率及p53和NF-κB的表达均显著升高(P<0.05);与H2O2组比较,pcDNA3.1-Corin组细胞活力及ki67和IκBα的表达均显著升高,凋亡率及p53和NF-κB的表达均显著降低(P<0.05);与pcDNA3.1-Corin组比较,pcDNA3.1-Corin+BAY 11-7082组细胞活力及ki67和IκBα的表达均显著升高,凋亡率及p53和NF-κB的表达均显著降低(P<0.05)。结论过表达Corin可通过抑制NF-κB信号通路提高心肌细胞活力及降低细胞凋亡。Objective To discuss the influence of Corin gene on proliferation and apoptosis of cardiomyocytes through nuclear factor-κB(NF-κB)signaling pathway.Methods Overexpressed Corin was transfected into H9c2 cells and H2O2 stimulating cells by LipofectamineTM2000,and all cells were divided into control group,pcDNA3.1 group,H2O2 group(treated with H2O2 in 150μmol/L for 6 h)and pcDNA3.1-Corin group(transfected with pcDNA3.1-Corin then treated with H2O2 for 6 h).Taken BAY 11-7082 as the inhibitor of NF-κB signaling pathway,cell activity was detected by using CCK8 method,and apoptosis rate was detected by using flow cytometry.The protein expressions of ki67(proliferation-related protein),p53(apoptosis-related protein),and NF-κB and IκBαin NF-κB signaling pathway were detected by using Western blotting assay.Results The expression of Corin decreased significantly in H9c2 cells stimulated with H2O2,and increased significantly after overexpressed.The cell activity and expressions of ki67 and IκBαdecreased significantly,and apoptosis rate and expressions of p53 and NF-κB increased significantly in H2O2 group compared with pcDNA3.1 group(P<0.05).The cell activity and expressions of ki67 and IκBαincreased significantly,and apoptosis rate and expressions of p53 and NF-κB decreased significantly in pcDNA3.1-Corin group compared with H2O2 group(P<0.05).The cell activity and expressions of ki67 and IκBαincreased significantly,and apoptosis rate and expressions of p53 and NF-κB decreased significantly in pcDNA3.1-Corin+BAY 11-7082 group compared with pcDNA3.1-Corin group(P<0.05).

关 键 词：Corin基因 心肌细胞 核转录因子-κB信号通路 增殖 凋亡 

分 类 号：R542.2[医药卫生—心血管疾病]