细胞膜色谱研究进展及其在中药活性成分筛选中的应用  被引量:17

Progress of Cell Membrane Chromatography and Its Application in Screening Active Ingredients of Traditional Chinese Medicine

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作  者:王晓宇 陈啸飞 顾妍秋 曹岩 原永芳 洪战英 柴逸峰 WANG Xiao-Yu;CHEN Xiao-Fei;GU Yan-Qiu;CAO Yan;YUAN Yong-Fang;HONG Zhan-Ying;CHAI Yi-Feng(East School of Pharmacy,Second Military Medical University,Shanghai 200433,China;Department of Pharmacy,Shanghai 9th People′s Hospital,Shanghai 201999,China)

机构地区:[1]第二军医大学药学院药物分析学教研室,上海市药物(中药)代谢产物研究重点实验室,上海200433 [2]上海市第九人民医院北院药剂科,上海201999

出  处:《分析化学》2018年第11期1695-1702,共8页Chinese Journal of Analytical Chemistry

基  金:国家自然科学基金项目(Nos.81503039,81573396,81673386);上海市扬帆青年人才计划(No.15YF1400200)资助。

摘  要:细胞膜色谱法作为近年来发展迅速的生物色谱技术,已成为筛选中药复杂体系中活性成分和研究药物与受体相互作用的重要工具。本文综述了细胞膜色谱技术的发展、分离原理、制备方法、针对其稳定性和寿命等问题进行的制备方法学优化、多种二维色谱方法学和应用策略,以及在中药等复杂体系活性成分筛选中的应用情况。在此基础上,本文展望了细胞膜色谱技术未来的发展趋势,以期为细胞膜色谱技术的进一步发展以及在药物高通量筛选领域的拓展应用提供思路。Cell membrane chromatography,which has been used to build an in vitro screening model,has become a practical method to screen active ingredients of traditional Chinese medicine or to study the interaction between drugs and receptors.In this paper,the development of cell membrane chromatography technology and its preparation methods,the optimization of the system and methodological for its stability and other issues were reviewed,as well its unique advantages on selection of active ingredients of traditional Chinese medicine.On this basis,the future development trend of cell membrane chromatography technology was discussed in order to provide ideas for the further development of cell membrane chromatography and its further application in the field of drug high throughput screening.

关 键 词:细胞膜色谱法 共价制备策略 全二维细胞膜色谱分析系统 中药活性成分筛选 评述 

分 类 号:R284[医药卫生—中药学] O657.7[医药卫生—中医学]

 

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