鼻敏感颗粒对变应性鼻炎大鼠模型细胞因子的影响  

Effect of Bi-Min-Gan granule on cytokines in rats with allergic rhinitis

作　　者：陈琴[1] 黄益灯[1] CHEN Qin;HUANG Yi-deng(Wenzhou Medical University,Wenzhou 325000,China)

机构地区：[1]温州医科大学,浙江温州325000

出　　处：《基础医学与临床》2018年第11期1553-1557,共5页Basic and Clinical Medicine

摘　　要：目的探讨鼻敏感颗粒对变应性鼻炎(AR)大鼠模型细胞因子的影响。方法将大鼠随机分为对照组、模型组(采用卵清蛋白为致敏原构建变异性鼻炎大鼠模型)、氯雷他定组[(10 g/(kg·d),灌胃治疗]和鼻敏感颗粒组[(10 g/(kg·d),灌胃治疗],每组各10只。ELISA检测细胞因子IL-1β、IL-17、IL-18和TGF-β1水平。采用Western blot和q-PCR检测各组T-bet和GATA-3蛋白和基因表达。结果与对照组比较,模型组IL-1β、IL-17和IL-18水平升高,TGF-β1水平降低(P<0. 05);与模型组比较,鼻敏感颗粒组和氯雷他定组IL-1β、IL-17和IL-18水平降低,TGF-β1水平升高(P<0. 05);与对照组比较,模型组GATA-3蛋白和基因表达水平升高,T-bet蛋白和基因表达水平降低(P<0. 05);与模型组比较,鼻敏感颗粒组和氯雷他定组GATA-3蛋白和基因表达水平降低,T-bet表达蛋白和基因水平升高(P<0. 05)。结论鼻敏感颗粒能够缓解AR大鼠鼻部炎性反应,其作用机制可能是下调GATA-3表达,上调T-bet表达,进而调控Th1/Th2细胞平衡。Objective To investigate the effect of Bi-Min-Gan granule on cytokines in rat model of allergic rhinitis.Methods Rats were randomly divided into control group,model group(using ovalbumin as a allergen to construct a rat model of variant rhinitis),loratadine group[10 g/(kg·d),gavage treatment]and nasal sensitivity.Bi-Min-Gan granule group[10 g/(kg·d),gavage treatment],10 rats in each group.The levels of IL-1β,IL-17,IL-18 and TGF-β1 were measured by ELISA before and after treatment.The expressions of T-bet and GATA-3 protein and gene expression after treatment in each group were detected by Western blot and q-PCR.Results Compared with the control group,the level of IL-1β,IL-17 and IL-18 in the model group were increased and the level of TGF-β1 was decreased(P<0.05).Compared with the model group,IL-1β,IL-17 and IL-18 decreased and TGF-β1 increased in loratadine group and Bi-Min-Gan granule group(P<0.05).Compared with the model group,GATA-3 protein and gene expression increased and T-bet protein and gene expression decreased(P<0.05).Compared with the model group,GATA-3 protein and gene expression decreased,the expression of T-bet protein and gene level increased in loratadine group and Bi-Min-Gan granule group(P<0.05).Conclusions Bi-Min-Gan granule can relieve nasal inflammation in allergic rhinitis rats.Its possible mechanism is to downregulate the expression of GATA-3,up-regulate the expression of T-bet,and then regulate the balance of Th1/Th2 cells.

关 键 词：鼻敏感颗粒 变应性鼻炎大鼠 细胞因子 GATA-3 T-BET 

分 类 号：R765.22[医药卫生—耳鼻咽喉科]