大花序桉组培快繁技术及其无性系造林效果的初步评价  

Tissue Culture Techniques for Eucalyptus cloeziana and Field Evaluation Resultant Clones

作　　者：唐再生 王建忠 莫继有 熊涛 张磊 李丽芳 兰俊 TANG Zaisheng;WANG Jianzhong;MO Jiyou;XIONG Tao;ZHANG Lei;LI Lifang;LAN Jun(Guangxi Dongmen State-owned Forest Farm,Fusui 532108,Guangxi,China)

机构地区：[1]广西国有东门林场,广西扶绥532108

出　　处：《桉树科技》2018年第3期36-45,共10页Eucalypt Science & Technology

基　　金：广西林业科技项目"大花序桉良种组培快繁技术研究"(桂林科研[2015]第18号)

摘　　要：采用16~30 a生大花序桉优树伐桩萌芽,经无菌芽诱导、丛芽增殖、继代培养、生根培养、甁苗移栽、组培苗造林等技术环节的研究,筛选出一组适合大花序桉生长的培养基,建立以芽繁芽的组培快繁体系,获得实用的移栽和造林方法,形成一整套可面向规模化生产和推广造林的大花序桉组培快繁技术,其中无性系CL1的生根率达到88.9%,甁苗移栽成活率达到82.7%,试生产组培苗10万株,出圃造林近70 hm2,初具组培苗工厂化生产规模;对幼林观测测定结果表明:遗传性状稳定,再生植株能保持原株的优良遗传性状,林相整齐,速生丰产效果显著。Buds from 16 to 30 year old elite trees of Eucalyptus cloeziana were used to study aspects of tissue culture propagation of clones of these trees,including:sterile bud induction,cluster bud proliferation,successive transfer culture,in vitro rooting,transplanting into nursery growing tubes and early field establishment.In this study suitable tissue culture media for growth of E.cloeziana was identified along with protocols necessary for rapid in vitro tissue culture propagation,starting from buds collected off the original trees.These protocols,along with methods developed for subsequent nursery culture,can provide for rapid propagation of E.cloeziana elite trees and are suited to the needs of large-scale production to service plantation establishment programs.The rooting rate of the clone CL1 reached 88.9%,transplanting survival rate reached 82.7%and initial test production yielded one hundred thousand tissue culture seedlings,enough for establishment of 70 hm2 of commercial plantations.Early results from field trials show that the E.cloeziana tissue cultured clones can keep the superior traits of the original mother trees(i.e.the ortets),with the young clonal trees showing remarkably fast early growth.

关 键 词：大花序桉 组培快繁 无性系造林 

分 类 号：S722.37[农业科学—林木遗传育种]