SSeCKs与川崎病冠状动脉损伤关系的研究  

The relationship between SSeCKs and coronary artery injury in Kawasaki disease

作　　者：钱碧莹 秦婷婷 程铭烨 黄铧 赵建美[1] QIAN Biying;QIN Tingting;CHENG Mingye;HUANG Hua;ZHAO Jianmei(Department of Pediatrics,the Affiliated Hospital of Nantong University,Nantong 226001)

机构地区：[1]南通大学附属医院儿科,南通226001

出　　处：《南通大学学报（医学版）》2018年第4期256-259,共4页Journal of Nantong University(Medical sciences)

基　　金：南通市科技计划项目(MS22015071);江苏省研究生科研与实践创新计划项目(SJCX17-0644);江苏省妇幼健康科研项目(F201751)

摘　　要：目的 :观察幼兔川崎病模型中Src抑制的蛋白激酶C的底物(Src suppressed C kinase substrates, SSeCKs)的含量变化。方法:建立川崎病新西兰幼兔模型,运用热源仪测定其注射药物后的直肠温度;实时定量聚合酶链式反应(real-time quantitative polymerase chain reaction, RT-PCR)测定外周血单个核细胞SSeCKs mRNA的表达;免疫组化法测定冠状动脉切片SSeCKs的表达。结果:注射牛血清白蛋白后连续0.5 h检测体温,实验组幼兔体温有升高趋势,正常组无变化。连续5 d同一时间点检测体温差异无统计学意义。免疫组化结果显示,正常组、急性期组和恢复期组的SSeCKs阳性表达率分别为(5.85±1.01)%、(24.88±0.25)%和(8.20±0.54)%。急性期组高于正常组和恢复期组(P<0.05),恢复组略高于正常组(P<0.05)。正常组、急性期组和恢复期组外周血单个核细胞SSeCKs mRNA水平分别为1.00±0.15、6.59±0.34和2.39±0.30,急性期组明显高于正常组和恢复期组(P<0.05),恢复期组高于正常组(P<0.05)。结论:SSeCKs可能与川崎病冠状动脉损伤有关。Objective:To observe the changes of Src suppressed C kinase substrate(SSeCKs)in a rabbit Kawasaki disease model.Methods:The Kawasaki disease New Zealand young rabbit model was established.The rectal temperature after injection of the drug was measured using a heat source meter.The expression of SSeCKs mRNA in peripheral blood mononuclear cells was determined by real-time quantitative polymerase chain reaction(RT-PCR).Immunohistochemistry was used to study the expression of SSeCKs in coronary artery sections.Results:Rabbits injected with bovine serum albumin had a tendency to increase in body temperature,while those in the normal group had no change.And rectal temperature was no significant difference at the same time for 5 consecutive days.In the immunohistochemical,the positive expression levels of SSeCKs in the normal group,the severe phase group and the recovery phase group were(5.85±1.01)%,(24.88±0.25)%and(8.20±0.54)%,respectively.The severe phase group was higher than the normal and recovery phase groups(P<0.05),and the recovery group was slightly higher than the normal group.The difference was statistically significant(P<0.05).The SSeCKs mRNA levels in peripheral blood mononuclear cells in the normal,severe,and recovery phase were 1.00±0.15,6.59±0.34,and 2.39±0.30,respectively,which were significantly higher in the acute phase group than in the normal group and the recovery group.And the recovery group was higher than the normal group.The difference was statistically significant(P<0.05).Conclusion:SSeCKs may be associated with coronary artery injury in Kawasaki disease.

关 键 词：川崎病 幼兔模型 Src抑制的蛋白激酶C的底物 

分 类 号：R725.5[医药卫生—儿科]