几种杀虫剂对烟青虫抗氧化酶的影响  

Effects of several insecticides on antioxidase of Heliothis assulta

作　　者：孟令玉 朱承美[1] 刘世涛[1] 曲爱军[1] 温亚萌 MENG Lingyu;ZHU Chengmei;LIU Shitao;QU Aijun;WEN Yameng(College of Plant Protection,Shandong Agricultural University,Taian 271018,Shandong,China)

机构地区：[1]山东农业大学植物保护学院,山东泰安岱宗大街61号271018

出　　处：《中国烟草学报》2018年第5期137-140,共4页Acta Tabacaria Sinica

摘　　要：为探讨6种常用杀虫剂对烟青虫抗氧化酶活性的影响,以烟青虫3龄和4龄幼虫为材料,应用愈创木酚法等方法测定了溴氰菊酯、高效氯氰菊酯、阿维菌素、乙酰甲胺磷、甲维盐和氯虫苯甲酰胺6种杀虫剂对烟青虫3龄和4龄幼虫体内超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GPX)酶类活性的影响。实验结果证实,经6种杀虫剂处理后,烟青虫3龄和4龄幼虫体内的抗氧化酶活性均高于对照,并且不同龄期的烟青虫体内各种酶活性变化趋势一致,但4龄幼虫酶活性增长率低于3龄幼虫。表明供试杀虫剂对3龄和4龄幼虫体内酶活性均能产生一定的影响,并且4龄幼虫对农药的敏感性低于3龄幼虫。Effects of six insecticides,i.e.deltamethrin,beta-cypermethrin,abamectin,acephate,emamectin benzoate,chlorantraniliprole,on activity of superoxide dismutase(SOD),peroxidase(POD),catalase(CAT)and glutathione peroxidase(GPX)in third and fourth instar larvae of H.assulta were determined by guaiacol method.Experimental results showed that the activity of antioxidase in third and fourth instar larvae of H.assulta was higher than control group under six kinds of insecticides stress and the variation trend of various enzyme activities in third and fourth instar larvae of H.assulta was consistent.Results also showed that the tested insecticides could have a certain eff ect on enzyme activity of third and fourth instar larvae and the sensitivity of fourth instar larvae to insecticides was lower than that of third instar larvae.

关 键 词：杀虫剂胁迫 烟青虫 抗氧化酶 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]