微生物絮凝剂MBFA9对小球藻的沉降作用及其机理  

Harvesting of microbial flocculant MBFA9 on Chlorella vulgaris and its mechanism

作　　者：张佳琪[1] 曹琬 郑广泰 姜彬慧[1] ZHANG Jiaqi;CAO Wan;ZHENG Guangtai;JIANG Binhui(School of Resources and Civil Engineering,Northeastern University,Shenyang 110819,China)

机构地区：[1]东北大学资源与土木工程学院,辽宁沈阳110819

出　　处：《中南大学学报（自然科学版）》2018年第10期2636-2642,共7页Journal of Central South University:Science and Technology

基　　金：国家科技重大专项(2013ZX07202-010-05);国家自然科学基金资助项目(51278090)~~

摘　　要：以小球藻为研究对象,对絮凝菌类芽孢杆菌A9(Paebubacillussp.A9)所产生的微生物絮凝剂MBFA9沉降水中微藻的性能进行研究。通过Zeta电位、结合键分析和扫描电镜表征探究MBFA9对小球藻的沉降机理。研究结果表明MBFA9对小球藻具有较好的沉降作用,其最佳沉降条件为:在小球藻对数生长期及平稳期添加Ca(OH)2调节pH至9.5,MBFA9添加量(体积分数)为0.2%(质量浓度为6 mg/L),在此最佳沉降条件下,沉降率为82.67%;随着pH升高,Zeta电位相应上升,在pH=9.5时达到最大;投加MBFA9后,分散状态的块状小球藻细胞迅速结合成网状结构,形成大的凝聚体而沉积,从而增大沉降速率。The characteristic and mechanism of harvesting Chlorella vulgaris by microbial flocculant MBFA9 which was produced by flocculation bacteria(Paebubacillus sp.A9)were studied.The sedimentation mechanism of Chlorella vulgaris by MBFA9 was investigated by Zeta potential,binding bond analysis and scanning electron microscopy.The results show that the sedimentation rate is 82.67%under the optimum settling conditions,i.e.adjusting pH=9.5 by adding Ca(OH)2 during exponential growth period and stationary period,and the additive volume fraction of MBFA9 is 0.1%.With the increase of pH,the Zeta potential increases accordingly and reaches the maximum when pH=9.5.After adding MBFA9,the dispersing state of Chlorella vulgaris cells combine rapidly into a network structure to form large agglomerates and deposite,leading to the increase of the sedimentation rate.

关 键 词：小球藻 PH 微生物絮凝剂 沉降作用 

分 类 号：X501[环境科学与工程—环境工程]