福建省沙门菌毒力基因sitC及iroN分布特征分析  

Distribution features of Salmonella virulence genes sitC and iroN in Fujian,China

作　　者：黄梦颖[1,2] 陈建辉 邱玉锋[1] 杨劲松 陈爱平[1,2] HUANG Meng-ying;CHEN Jian-hui;QIU Yu-feng;YANG Jin-song;CHEN Ai-ping(Fujian Center for Disease Control and Prevention,,Fuzhou 350001,China;Fujian province Key Laboratory Zoonosis Research,Fuzhou 350001,China)

机构地区：[1]福建省疾病预防控制中心,福州350001 [2]福建省人兽共患病研究重点实验室,福州350001

出　　处：《中国人兽共患病学报》2018年第9期783-787,共5页Chinese Journal of Zoonoses

基　　金：福建省医学创新课题(No.2011-CXB-19)资助~~

摘　　要：目的比较福建省腹泻患者与健康人群分离的沙门菌毒力基因sitC及iroN分布特征;同时分析两种毒力基因在福建省分离率较高的4种常见血清型沙门菌中的分布差异,为进一步有效防控沙门菌病提供依据。方法选择福建省从腹泻患者和健康人群中分离到的沙门菌166株,其中腹泻患者121株、健康人群45株,包括鼠伤寒、肠炎、德尔卑和斯坦利四种血清型,运用常规PCR方法扩增毒力基因sitC及iroN。结果毒力基因sitC及iroN在166株沙门菌分离株中的总检出率分别为51.81%(86株)和39.76%(66株)。在腹泻患者和健康人群两个组别中,sitC及iroN的检出率差异无统计学意义(χ_(sitC)~2=0.347,P=0.556;χ_(iroN)~2=3.322,P=0.068)。在鼠伤寒、肠炎、德尔卑和斯坦利四种血清型中,sitC及iroN的检出率差异有统计学意义(χ_(sitC)~2=11.634,P=0.009;χ_(iroN)~2=15.020,P=0.002)。sitC+iroN+及sitC-iroN-是最常见的基因携带模式。结论福建省沙门菌分离株中均检出了一定比例的sitC和iroN毒力基因。这两个毒力基因的分布存在血清型差异,但在健康人群和腹泻病人的沙门菌分离株中不存在分布差异。所以健康人群的沙门菌分离株与腹泻病人的沙门菌分离株一样都具有较强的生存能力和一定的致病潜能。We aimed to find out the distribution features of virulence genes sitC and iroN of Salmonella enterica isolates from diarrhea patients and healthy people in Fujian,and analyse the distribution differences of these virulence genes in the four common serotypes of Salmonella enteric in Fujian,so as to provide the basis for the further effective prevention and control of salmonellosis.A total of 166 strains of Salmonella enterica isolated from diarrhea patients(121 strains)and healthy people(45 strains)in Fujian Province were selected,including four serotypes of S.typhimus,S.enteritis,S.Del humiliation and S.Stanley.The conventional PCR method was used to amplify the virulence gene sitC and iroN.SPSS 17 was used for data statistics.Results showed that the total detection rates of virulence genes sitC and iroN in 166 isolated strains were 51.81%(86 strains)and 39.76%(66 strains),respectively.There was no significant difference in the detection rate of sitC and iroN in diarrhea patients and healthy people(χ2 sitC=0.347,P=0.556;χ2 iroN=3.322,P=0.068).While there was significant difference in the detection rate of sitC and iroN in the four serotypes of S.typhimus,S.enteritis,S.Del humiliation and S.Stanley(χ2 sitC=11.634,P=0.009;χ2 iroN=15.020,P=0.002).SitC+iroN+and sitC-iroN-were the most common patterns of gene delivery.A certain proportion of sitC and iroN were detected in Salmonella isolates from Fujian Province.The distribution of these two virulence genes was different from serotypes,but there was no difference in the distribution of Salmonella isolates between healthy people and diarrhea patients.So the Salmonella isolates from healthy people in Fujian Province have strong survival ability and certain pathogenicity potential,as well as Salmonella isolates from diarrhea patients.

关 键 词：沙门菌 毒力基因 腹泻患者 健康人群 血清型 

分 类 号：R378.2[医药卫生—病原生物学]