四川省鼠疫耶尔森菌CRISPR基因分型及地区分布研究  

CRISPR genotyping and regional distribution of Yersinia pestis in Sichuan Province,China

作　　者：祁腾[1] 梁莹[2] 李伟[2] 杨军[3] 罗隆泽[1] 段风刚 汪立茂[1] 李帆[1] 金忠强 谭文明[4] 谢飞[4] 王宏[5] 刀吉 刘建 曾林子[1] 廖虹瑜[1] 雷高鹏[1] QI Teng;LIANG Ying;LI Wei;YANG Jun;LUO Long-ze;DUAN Feng-gang;WANG Li-mao;LI Fan;JIN Zhong-qiang;TAN Wen-ming;XIE Fei;WANG Hong;DAO Ji;LIU Jian;ZENG Lin-zi;LIAO Hong-yu;LEI Gao-peng(Sichuan Center for Disease Control and Prevention,Chengdu 610041,China;China Center for Disease Control and Prevention,Beijing 102206,China;Ganzi Tibeian Autonomous Prefecture Center for Disease Control and Prevention,Kangding 626000,China;Shiqu Center for Disease Control and Prevention,Shiqu 627350,China;Dege Center for Disease Control and Prevention,Dege 627250,China;Litang Center for Disease Control and Prevention,Litang 624300,China;Batang Center for Disease Control and Prevention,Batang 627650,China)

机构地区：[1]四川省疾病预防控制中心,成都610041 [2]中国疾病预防控制中心,北京102206 [3]甘孜州疾病预防控制中心,康定626000 [4]石渠县疾病预防控制中心,石渠627350 [5]德格县疾病预防控制中心,德格627250 [6]理塘县疾病预防控制中心,理塘624300 [7]巴塘县疾病预防控制中心,巴塘627650

出　　处：《中国人兽共患病学报》2018年第9期801-804,810,共5页Chinese Journal of Zoonoses

基　　金：四川省卫计委课题(No.16PJ397)资助~~

摘　　要：目的对四川省鼠疫耶尔森菌(Yersinia pestis)做规律成簇的间隔短回文重复序列(Clustered regularly interspaced short palindromic repeats CRSIPR)基因分型和地区分布研究,为四川鼠疫防治提供科学依据。方法对132株鼠疫菌培养和提取核酸,应用3对CRISPR引物对被试菌株DNA进行PCR扩增、测序和分析,确定四川省鼠疫菌的CRSIPR基因型。结果 132株鼠疫菌共发现17种spacer,包括YPa 9种、YPb 5种、YPc 3种,新发现的1种space阵列。所有鼠疫菌株被分为2个CRISPR基因簇(Cc3′,Ca7),3个基因型(37′型、22型和sc01型)。其中石渠县青海田鼠型菌株基因型为37′型,石渠县人间鼠疫的菌株为22型,德格县旱獭型菌株基因型为22型,巴塘县、理塘县、雅江县和新龙县旱獭菌株基因型为sc01型。结论四川省鼠疫菌菌株具有明显的地区分布特征。We studied the CRISPR genotypes data and regional distribution of Y.pestis strains in Sichuan Province,and provided a scientific basis for prevention and treatment of plague.For determining the CRSIPR genotype,we cultivated and extracted nucleic acid from 132 Y.pestis strains which isolated from plague foci of Sichuan Province.Using PCR amplification,sequencing and analysis,all strains were genotyped with YPa,YPb and YPc primers of CRISPR.Results showed that we found 17 spacers,including 9 of YPa,5 of YPb and 3of YPc,and a newly kind of space array was discovered in 132 strains of Y.pestis.All pathogens were divided into 2 CRISPR gene clusters(Cc3′,Ca7)and 3 genotypes(37′,22 and sc01Type).Microtus fuscus strains were genotype 37′,and a strain isolated from plague patients in Shiqu county was genotype 22,and Marmota himalayana strains were genotype 22 in Dege County,and Marmota himalayana strains were genotype sc01 in Batang County,Litang County,Yajiang County and Xinlong County.Strains of Y.pestis in Sichuan Province have a strongly regional distribution characteristics.

关 键 词：鼠疫 四川省 CRISPR 分子分型 

分 类 号：Q786[生物学—分子生物学] S855.2[农业科学—临床兽医学]